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Pub. No.:    WO/2006/083516    International Application No.:    PCT/US2006/001107
Publication Date: 10.08.2006 International Filing Date: 12.01.2006
A61K 39/00 (2006.01), A61K 39/02 (2006.01), A61K 39/385 (2006.01), A61K 39/295 (2006.01)
Applicants: OSEL, INC. [US/US]; 4008 Burton Drive, Santa Clara, California 95054 (US) (For All Designated States Except US).
LIU, Xiaowen [CN/US]; (US) (For US Only).
ESSENMACHER, Kirsten [US/US]; (US) (For US Only).
SIMPSON, David A. [CA/US]; (US) (For US Only).
XU, Qiang [US/US]; (US) (For US Only)
Inventors: LIU, Xiaowen; (US).
SIMPSON, David A.; (US).
XU, Qiang; (US)
Agent: GAO, Chuan; Townsend and Townsend and Crew LLP, 2 Embarcadero Center, 8th Floor, San Francisco, California 94111 (US)
Priority Data:
60/643,613 12.01.2005 US
Abstract: front page image
(EN)This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed Lactobacillus bacterium. In addition, this disclosure describes two novel promoters originated from L. jensenii, which are capable of directing a high level of gene expression in Lactobacillus bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified Lactobacillus bacterium containing the expression cassette, a method for recombinant gene expression in Lactobacillus bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.
(FR)Version modifiée de protéine de cyanovirine-N et bactérie Lactobacillus transformée capable d'induire l'expression en recombinaison de cette protéine modifiée. Séquence polynucléotidique codant ladite protéine modifiée, et procédé d'élaboration et d'utilisation de cette protéine et de la bactérie en question. Par ailleurs, deux promoteurs issus L. jensenii, capables d'induire un niveau élevé d'expression génique dans les bactéries Lactobacillus. Egalement, cassette d'expression renfermant l'un des promoteurs considérés, bactérie Lactobacillus génétiquement modifiée contenant la cassette en question, procédé d'expression génique en recombinaison dans les bactéries Lactobacillus reposant sur l'utilisation des promoteurs décrits, et procédé de délivrance de protéines ayant l'activité biologique souhaitée sur une surface de muqueuse humaine.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)