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1. (WO2006083382) MEASURING FORSTER RESONANCE ENERGY TRANSFER WITH POLARIZED AND DEPOLARIZED LIGHT
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/083382    International Application No.:    PCT/US2005/043351
Publication Date: 10.08.2006 International Filing Date: 01.12.2005
IPC:
G01N 33/68 (2006.01), G01N 21/64 (2006.01), C12M 1/34 (2006.01)
Applicants: VANDERBILT UNIVERSITY [US/US]; Box 6009, Station B, Nashville, TN 37235 (US) (For All Designated States Except US).
PISTON, David, W. [US/US]; (US) (For US Only).
RIZZO, Mark, A. [US/US]; (US) (For US Only)
Inventors: PISTON, David, W.; (US).
RIZZO, Mark, A.; (US)
Agent: HIGHLANDER, Steven, L.; Fulbright & Jaworski L.L.P., 600 Congress Avenue,, Suite 2400, Austin, TX 78701 (US)
Priority Data:
60/632,668 02.12.2004 US
Title (EN) MEASURING FORSTER RESONANCE ENERGY TRANSFER WITH POLARIZED AND DEPOLARIZED LIGHT
(FR) MESURE DE TRANSFERT D'ENERGIE PAR RESONANCE DE TYPE FORSTER A L'AIDE DE LUMIERE POLARISEE ET DEPOLARISEE
Abstract: front page image
(EN)The present application provides improved methods for assessing Förster resonance energy transfer (FRET) using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules. Depolarization of fluorescence emission is observed only in the presence of energy transfer. A simple detection strategy is described for fluorescence microscopy using laser scanning and wide field techniques. FRET between linked and unlinked green fluorescent proteins (GFPs) in living cells can be monitored with a larger dynamic range than in other techniques.
(FR)La présente invention porte sur des procédés améliorés permettant d'évaluer un transfert d'énergie par résonance de type Förster à l'aide de lumière polarisée. De manière plus spécifique, les procédés consistent à mesurer la lumière dépolarisée émise par des molécules accepteurs fluorescentes.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)