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1. (WO2006069403) METHODS FOR PRODUCING SOLUBLE MULTI-MEMBRANE-SPANNING PROTEINS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2006/069403 International Application No.: PCT/US2005/047653
Publication Date: 29.06.2006 International Filing Date: 22.12.2005
IPC:
C12N 15/70 (2006.01) ,C07K 14/705 (2006.01) ,C07K 16/18 (2006.01) ,G01N 33/53 (2006.01) ,G01N 33/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70
Vectors or expression systems specially adapted for E. coli
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
ERNST, James, A. [US/US]; US (UsOnly)
YANSURA, Daniel, G. [US/US]; US (UsOnly)
KIM, Hok, Seon [KR/US]; US (UsOnly)
GENENTECH, INC. [US/US]; 1 Dna Way South San Francisco, CA 94080-4990, US (AllExceptUS)
Inventors:
ERNST, James, A.; US
YANSURA, Daniel, G.; US
KIM, Hok, Seon; US
Agent:
KOWALCHYK, Katherine, M.; Merchant & Gould P.C P.O. Box 2903 Minneapolis, MN 55402-0903, US
Priority Data:
60/639,23322.12.2004US
Title (EN) METHODS FOR PRODUCING SOLUBLE MULTI-MEMBRANE-SPANNING PROTEINS
(FR) PROCEDES POUR LA PRODUCTION D'UNE PLURALITE DE PROTEINES TRANSMEMBRANAIRES SOLUBLES
Abstract:
(EN) Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.
(FR) La présente invention a trait à des procédés pour la production de protéines transmembranaires en rendements élevés, à conformation native, et/ou en forme soluble comprenant la solubilisation dans des détergents non ioniques ou zwitterrioniques ainsi que l'utilisation de promoteurs et de vecteurs d'expression pour l'expression à rendements élevés de polypeptides transmembranaires dans des cellules bactériennes. Des promoteurs mutés assurent une contrôle strict sur des polypeptides transmembranaires dans des hôtes de cellules bactériennes
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)