WO2006055836 IN VIVO ALTERATION OF CELLULAR DNA

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Publication Number WO/2006/055836

Publication Date 26.05.2006

International Application No. PCT/US2005/041953

International Filing Date 16.11.2005

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Applicants

PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US] (AllExceptUS)
CHURCH, George, M. [US]/[US] (UsOnly)
REPPAS, Nikos [US]/[US] (UsOnly)

Inventors

CHURCH, George, M.
REPPAS, Nikos

Agents

IWANICKI, John, P.

Priority Data

60/628,211

16.11.2004

Publication Language English (en)

Filing Language English (EN)

Designated States

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Title

(EN) IN VIVO ALTERATION OF CELLULAR DNA

(FR) ALTERATION IN VIVO DE L'ADN CELLULAIRE

Abstract

(EN) The present invention relates to methods, cells and compositions for performing in vivo recombination without the need for clonal selection. These methods, cells and compositions are useful for the automation of recombination. The present invention provides methods for performing sequential steps in genome engineering, as well as for achieving parallel and multiplex genome engineering.

(FR) La présente invention concerne des méthodes, des cellules et des compositions destinées à la réalisation d'une recombinaison in vivo sans avoir recours à une sélection clonale. Ces méthodes, cellules et compositions sont utilisées dans l'automatisation de la recombinaison. Cette invention a aussi pour objet des méthodes de réalisation d'étapes séquentielles dans l'ingénierie génomique, ainsi que d'obtention d'une ingénierie génomique parallèle et multiplexe.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

A	HUMAN NECESSITIES
01	AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K	ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2217	Genetically modified animals
05	Animals comprising random inserted nucleic acids (transgenic)

A	HUMAN NECESSITIES
01	AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K	ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2217	Genetically modified animals
07	Animals genetically altered by homologous recombination
075	inducing loss of function, i.e. knock out

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
87	Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90	Stable introduction of foreign DNA into chromosome

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1. WO2006055836 - IN VIVO ALTERATION OF CELLULAR DNA