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1. WO2006053929 - METHOD OF PRODUCING THE PENICILLIN V ACYLASE ENZYME FROM STREPTOMYCES LAVENDULAE IN RECOMBINANT MICRO-ORGANISMS

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Publication Number WO/2006/053929
Publication Date 26.05.2006
International Application No. PCT/ES2005/070027
International Filing Date 15.03.2005
IPC
C12N 15/55 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
55Hydrolases (3)
C12N 9/14 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
C12N 1/21 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
CPC
C12N 9/84
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
78acting on carbon to nitrogen bonds other than peptide bonds (3.5)
80acting on amide bonds in linear amides ; (3.5.1)
84Penicillin amidase ; (3.5.1.11)
C12Y 305/01011
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
305Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
01in linear amides (3.5.1)
01011Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase
Applicants
  • ANTIBIÓTICOS, S.A.U. [ES]/[ES] (AllExceptUS)
  • ACEBAL SARABIA, Carmen [ES]/[ES] (UsOnly)
  • DE LA MATA RIESCO, Isabel [ES]/[ES] (UsOnly)
  • TORRES BACETE, Jesús [ES]/[ES] (UsOnly)
  • BARREDO FUENTE, José Luis [ES]/[ES] (UsOnly)
  • GARCÍA LOPEZ, José Luis [ES]/[ES] (UsOnly)
  • LOPEZ ORTÍZ, Juan Francisco [ES]/[ES] (UsOnly)
  • CASTILLÓN BORREGUERO, María Pilar [ES]/[ES] (UsOnly)
  • RODRÍGUEZ SÁIZ, Marta [ES]/[ES] (UsOnly)
  • MORENO VALLE, Miguel Ángel [ES]/[ES] (UsOnly)
  • ARROYO SÁNCHEZ, Miguel [ES]/[ES] (UsOnly)
  • TORRES GUZMAN, Raquel [ES]/[ES] (UsOnly)
  • CABRI, Walter [IT]/[ES] (UsOnly)
Inventors
  • ACEBAL SARABIA, Carmen
  • DE LA MATA RIESCO, Isabel
  • TORRES BACETE, Jesús
  • BARREDO FUENTE, José Luis
  • GARCÍA LOPEZ, José Luis
  • LOPEZ ORTÍZ, Juan Francisco
  • CASTILLÓN BORREGUERO, María Pilar
  • RODRÍGUEZ SÁIZ, Marta
  • MORENO VALLE, Miguel Ángel
  • ARROYO SÁNCHEZ, Miguel
  • TORRES GUZMAN, Raquel
  • CABRI, Walter
Agents
  • ELZABURU, Alberto De
Priority Data
20040065016.03.2004ES
Publication Language Spanish (es)
Filing Language Spanish (ES)
Designated States
Title
(EN) METHOD OF PRODUCING THE PENICILLIN V ACYLASE ENZYME FROM STREPTOMYCES LAVENDULAE IN RECOMBINANT MICRO-ORGANISMS
(ES) PROCEDIMIENTO PARA PRODUCIR LA ENZIMA PENICILINA V ACILASA DE STREPTOMYCES LAVENDULAE EN MICROORGANISMOS RECOMBINANTES.
(FR) PROCEDE DESTINE A PRODUIRE L'ENZYME PENICILLINE V ACYLASE DE STREPTOMYCES LAVENDULAE DANS DES MICROORGANISMES RECOMBINANTS
Abstract
(EN) The invention relates to a method of producing the penicillin V acylase enzyme from Streptomyces lavendulae in recombinant micro-organisms. The inventive enzyme production method comprises the following steps: (1) identification of the DNA corresponding to the pva gene, which encodes the penicillin V acylase enzyme from S. lavendulae ATCC 13664, (2) cloning of said gene in a vector suited to the host cell used, (3) cultivation of recombinant host cells bearing the pva gene, and (4) recovery of the enzyme from the cell culture. The PVA enzyme thus obtained is used in the individual production of ß-lactamic antibiotics, particularly 6-aminopenicillanic (6-APA) acid from penicillin V, although other penicillins (K, F, and dihydro-F) can also be used as a substrate.
(ES) Procedimiento para producir la enzima penicilina V acilasa de Streptomyces lavendulae en microorganismos recombinantes . El procedimiento de producción de la enzima comprende: (1) identificación del ADN correspondiente al gen pva, el cual codifica la enzima penicilina V acilasa de 5. lavendulae ATCC 13664, (2) clonación del citado gen en un vector apropiado para la célula hospedadora utilizada, (3) cultivo de las células hospedadoras recombinantes portadoras del gen pva y (4) recuperación de la enzima a partir del cultivo de las células. La enzima PVA obtenida se utiliza en la producción individual de antibióticos &bgr;-lactámicos, particularmente del ácido 6-aminopenicilánico (6 -APA) a partir de penicilina V, pero también puede utilizar otras penicilinas (K, F, y dihidro F) como sustrato.
(FR) L'invention concerne un procédé destiné à produire l'enzyme pénicilline V acylase de Streptomyces lavendulae dans des microorganismes recombinants. Le procédé de production de cette enzyme consiste: (1) à identifier l'ADN correspondant au gène PVA, lequel codifie l'enzyme pénicilline V acylase de 5. Lavendulae ATCC 13664, (2) à cloner le gène cité en un vecteur approprié pour la cellule d'hébergement utilisée, (3) à cultiver les cellules d'hébergement recombinantes porteuses du gène PVA et (4) à récupérer l'enzyme à partir de la culture de cellules. L'enzyme PVA obtenue s'utilise dans la production individuelle d'antibiotiques ß-lactamiques, notamment de l'acide 6-aminopénicillanique (6 -APA) à partir de pénicilline V, mais peut également utiliser d'autres pénicillines (K, F, et dihydro F) en tant que substrat.
Related patent documents
ES1057822
Latest bibliographic data on file with the International Bureau
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