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1. WO2006048683 - TOTAL INTERNAL REFLECTANCE FLUORESCENCE (TIRF) MICROSCOPE

Publication Number WO/2006/048683
Publication Date 11.05.2006
International Application No. PCT/GB2005/004314
International Filing Date 08.11.2005
IPC
G01Q 60/22 2010.1
GPHYSICS
01MEASURING; TESTING
QSCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING-PROBE MICROSCOPY
60Particular types of SPM or apparatus therefor; Essential components thereof
18SNOM or apparatus therefor, e.g. SNOM probes
22Probes, their manufacture or their related instrumentation, e.g. holders
G02B 21/08 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
06Means for illuminating specimen
08Condensers
G02B 21/16 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination
CPC
G02B 21/082
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
06Means for illuminating specimens
08Condensers
082for incident illumination only
G02B 21/16
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
16adapted for ultra-violet illumination ; ; Fluorescence microscopes
Applicants
  • IMPERIAL INNOVATIONS LIMITED [GB]/[GB] (AllExceptUS)
  • FERENCZI, Michael, Alan [GB]/[GB] (UsOnly)
Inventors
  • FERENCZI, Michael, Alan
Agents
  • KILBURN & STRODE
Priority Data
0424652.608.11.2004GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TOTAL INTERNAL REFLECTANCE FLUORESCENCE (TIRF) MICROSCOPE
(FR) MICROSCOPE A FLUORESCENCE PAR REFLEXION INTERNE TOTALE (TIRF)
Abstract
(EN) A total internal reflection fluorescent (TIRF) microscope has a conjugate lens (30) which is positioned off of the primary microscope axis (AA). The lens (30) is held by a mount (60) allowing the radial spacing between the microscope axis (AA) and the conjugate lens axis (BB) to be varied. The mount (60) also allows the lens to be rotated around the microscope axis. As the lens (30) rotates, the spot of light (64) created by the incident laser beam moves around the periphery of the objective lens (34). This changes the direction of polarisation of the evanescent wave within which the sample (44) sits. By studying the emitted fluorescence as a function of lens angle, information is provided about the spatial attitude of a molecule under study.
(FR) L'invention se rapporte à un microscope à fluorescence par réflexion interne totale (TIRF) qui comporte une lentille conjuguée (30) placée en dehors de l'axe principal (AA) du microscope. La lentille (30) est maintenue par un support (60) permettant de faire varier l'espacement radial entre l'axe (AA) du microscope et l'axe (BB) de la lentille conjuguée. Le support (60) permet également de faire tourner la lentille autour de l'axe du microscope. Lorsque la lentille (30) tourne, la tache de lumière (64) créée par le faisceau laser incident se déplace autour de la périphérie de la lentille (34) de l'objectif. Ceci modifie le sens de polarisation de l'onde évanescente à l'intérieur de laquelle réside l'échantillon (44). En étudiant la fluorescence émise en fonction de l'angle de la lentille, on obtient des informations sur la position dans l'espace d'une molécule étudiée.
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