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1. WO2006046132 - METHOD FOR MODULATING THE EVOLUTION OF A POLYPEPTIDE ENCODED BY A NUCLEIC ACID SEQUENCE

Publication Number WO/2006/046132
Publication Date 04.05.2006
International Application No. PCT/IB2005/003317
International Filing Date 19.09.2005
IPC
C12N 15/10 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/01 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C12N 15/53 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
C12N 9/06 2006.1
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9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
06acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
C40B 10/00 2006.1
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
10Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
CPC
C12N 15/01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C12N 15/102
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
102Mutagenizing nucleic acids
C12N 15/1058
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
C12N 9/003
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0026acting on CH-NH groups of donors (1.5)
0028with NAD or NADP as acceptor (1.5.1)
003Dihydrofolate reductase [DHFR] (1.5.1.3)
C40B 10/00
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
10Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
G16B 30/00
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
30ICT specially adapted for sequence analysis involving nucleotides or amino acids
Applicants
  • INSTITUT PASTEUR [FR]/[FR] (AllExceptUS)
  • MAZEL, Didier [FR]/[FR] (UsOnly)
  • CAMBRAY, Guillaume [FR]/[FR] (UsOnly)
Inventors
  • MAZEL, Didier
  • CAMBRAY, Guillaume
Agents
  • MARTIN, Jean-Jacques
Priority Data
60/610,59717.09.2004US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR MODULATING THE EVOLUTION OF A POLYPEPTIDE ENCODED BY A NUCLEIC ACID SEQUENCE
(FR) PROCEDE PERMETTANT DE MODULER L'EVOLUTION D'UN POLYPEPTIDE CODE PAR UNE SEQUENCE D'ACIDES NUCLEIQUES
Abstract
(EN) A method for modulating the ability of a gene to mutate by analyzing codon usage within the gene and selecting a synonymous nucleotide sequence with a higher, lower or different capacity to mutate. The method permits widening and optimization of the evolutionary landscape of a protein. A computer-implemented method for analyzing and selecting nucleotide sequences with an altered ability to mutate.
(FR) Cette invention concerne un procédé permettant de moduler l'aptitude d'un gène à muter par analyse de l'utilisation du codon à l'intérieur du gène puis sélection d'une séquence nucléotidique synonyme présentant une capacité à muter supérieure, inférieure ou différente. Le procédé décrit dans cette invention permet d'élargir et d'optimiser le paysage évolutif d'une protéine. Cette invention concerne également un procédé informatisé permettant d'analyser et de sélectionner des séquences nucléotidiques présentant une aptitude à muter modifiée.
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