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1. WO2006044956 - METHODS FOR ASSEMBLY OF HIGH FIDELITY SYNTHETIC POLYNUCLEOTIDES

Publication Number WO/2006/044956
Publication Date 27.04.2006
International Application No. PCT/US2005/037571
International Filing Date 18.10.2005
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/66
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
Applicants
  • CODON DEVICES, INC. [US]/[US] (AllExceptUS)
  • AFEYAN, Noubar [CA]/[US] (UsOnly)
  • CHURCH, George [US]/[US] (UsOnly)
  • JACOBSON, Joseph [US]/[US] (UsOnly)
  • BAYNES, Brian, M. [US]/[US] (UsOnly)
  • NESMITH, Kenneth, Gabriel [US]/[US] (UsOnly)
  • CHAPMAN, Brad, Alan [US]/[US] (UsOnly)
  • STRACK-LOGUE, Bettina [DE]/[US] (UsOnly)
Inventors
  • AFEYAN, Noubar
  • CHURCH, George
  • JACOBSON, Joseph
  • BAYNES, Brian, M.
  • NESMITH, Kenneth, Gabriel
  • CHAPMAN, Brad, Alan
  • STRACK-LOGUE, Bettina
Agents
  • VINCENT, Matthew, P.
Priority Data
11/067,81228.02.2005US
11/068,32128.02.2005US
60/619,65018.10.2004US
60/643,81313.01.2005US
60/657,01428.02.2005US
60/698,56012.07.2005US
60/727,20514.10.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR ASSEMBLY OF HIGH FIDELITY SYNTHETIC POLYNUCLEOTIDES
(FR) PROCEDES D'ASSEMBLAGE DE POLYNUCLEOTIDES SYNTHETIQUES DE HAUTE FIDELITE
Abstract
(EN)
Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre­amplification of one or more oligonucleotides using 'universal' primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.
(FR)
L'invention concerne des procédés de fabrication d'ADN synthétiques, soit des ADN obtenus, du moins en grande partie, par synthèse chimique de polymères d'acides nucléiques. L'invention concerne également des procédés permettant d'assembler plusieurs ADN dans le même stock par assemblage multiplexé d'oligonucléotides synthétiques. Dans des modes de réalisation exemplaires, les procédés impliquent la préamplification d'au moins un oligonucléotide au moyen d'amorceurs 'universels', la réduction du taux d'erreur dans des produits d'oligonucléotides et/ou d'acides nucléiques, et l'optimisation séquentielle et la conception d'oligonucléotides. L'invention concerne enfin des réseaux d'acides nucléiques de faible pureté ainsi que des procédés d'assemblage d'acides nucléiques au moyen d'oligonucléotides obtenus de réseaux de faible pureté.
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