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1. WO2006040471 - PRODUCING HYDROGEN BY HETEROLOGOUS EXPRESSION OF A TYPE II NAD (P) H DEHYDROGENASE IN CHLAMYDOMONAS

Publication Number WO/2006/040471
Publication Date 20.04.2006
International Application No. PCT/FR2005/002511
International Filing Date 11.10.2005
IPC
C12P 3/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
3Preparation of elements or inorganic compounds except carbon dioxide
C12N 9/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
C12N 1/13 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
12Unicellular algae; Culture media therefor
13modified by introduction of foreign genetic material
C12N 15/53 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
CPC
C12N 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
12Unicellular algae; Culture media therefor
C12N 9/0036
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0012acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
0036acting on NADH or NADPH (1.6)
C12P 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
3Preparation of elements or inorganic compounds except carbon dioxide
C12Y 106/01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
106Oxidoreductases acting on NADH or NADPH (1.6)
01with NAD+ or NADP+ as acceptor (1.6.1)
Applicants
  • COMMISSARIAT A L'ENERGIE ATOMIQUE (CEA) [FR]/[FR] (AllExceptUS)
  • BERNARD, Laetitia [FR]/[FR] (UsOnly)
  • MUS, Florence [FR]/[FR] (UsOnly)
  • SIMON-DESPLATS, Carine [FR]/[FR] (UsOnly)
  • CUINE, Stéphan [FR]/[FR] (UsOnly)
  • COURNAC, Laurent [FR]/[FR] (UsOnly)
  • PELTIER, Gilles [FR]/[FR] (UsOnly)
Inventors
  • BERNARD, Laetitia
  • MUS, Florence
  • SIMON-DESPLATS, Carine
  • CUINE, Stéphan
  • COURNAC, Laurent
  • PELTIER, Gilles
Agents
  • VIALLE-PRESLES, Marie José
Priority Data
041071511.10.2004FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) PRODUCING HYDROGEN BY HETEROLOGOUS EXPRESSION OF A TYPE II NAD (P) H DEHYDROGENASE IN CHLAMYDOMONAS
(FR) PRODUCTION D'HYDROGENE PAR EXPRESSION HETEROLOGUE D'UNE NAD (P) H DESHYDROGENASE DE TYPE II CHEZ CHLAMYDOMONAS.
Abstract
(EN)
The invention concerns the use of a type II NAD(P)H dehydrogenase (NDH-II), or of a polynucleotide encoding said NDH-II, to increase the capacity of a green algae to produce hydrogen. Said polynucleotide is in particular useful for transforming said green algae, so as to improve its hydrogen production.
(FR)
La présente invention est relative à l'utilisation d'une NAD(P)H déshydrogénase de type II (NDH-II), ou d'un polynucléotide codant pour ladite NDH-II, pour augmenter la capacité d'une algue verte à produire de l'hydrogène. Ledit polynucléotide est notamment utilisable pour transformer ladite algue verte, afin d'améliorer sa production d'hydrogène.
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