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1. (WO2006034349) DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2006/034349 International Application No.: PCT/US2005/033838
Publication Date: 30.03.2006 International Filing Date: 21.09.2005
IPC:
C12Q 1/68 (2006.01) ,C07H 21/02 (2006.01) ,C07H 21/04 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02
with ribosyl as saccharide radical
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
Applicants:
POURMAND, Nader [SE/US]; US (UsOnly)
DAVIS, Ronald, W. [US/US]; US (UsOnly)
WANG, Shan, X. [US/US]; US (UsOnly)
THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY [US/US]; Stanford University Office of Technology Licensing 1705 El camino Real Palo Alto, CA 94306, US (AllExceptUS)
Inventors:
POURMAND, Nader; US
DAVIS, Ronald, W.; US
WANG, Shan, X.; US
Agent:
LUMEN IPS; 2345 Yale Street 2nd Floor Palo Alto, CA 94306, US
Priority Data:
60/612,00021.09.2004US
Title (EN) DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY
(FR) EMPREINTE GENETIQUE PAR ESSAI DE MIGRATION DE BRANCHE
Abstract:
(EN) A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
(FR) L'invention concerne un procédé de détermination de longueur de cible polynucléotidique, commençant par l'hybridation de cible à un jeu de premières sondes de différentes longueurs établies, pour donner la formation de duplex entre la cible polynucléotidique et les premières sondes. Ces duplex comportent une partie de cible à brin unique, si la cible est plus longue que la première sonde avec laquelle elle se trouve dans un duplex. Vient ensuite l'hybridation d'une seconde sonde de longueur établie à ces duplex. Si la longueur de la cible excède la longueur de la première sonde, on la déplace durant l'hybridation selon la technique de migration de branche. Au contraire, si ladite longueur est inférieure ou égale à celle de la première sonde, on ne la déplace pas. Ainsi, la longueur de la cible polynucléotidique peut être déterminée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)