WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2006020762) METHODS AND KITS FOR ASSAYING MYELOPEROXIDASE BY MEANS OF ENZYMATIC GLYCOLATE-GLYOXYLATE CYCLING REACTIONS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/020762    International Application No.:    PCT/US2005/028540
Publication Date: 23.02.2006 International Filing Date: 10.08.2005
Chapter 2 Demand Filed:    09.06.2006    
IPC:
C12Q 1/28 (2006.01)
Applicants: GENERAL ATOMICS [US/US]; 3550 General Atomics Court, P.O. Box 85608, San Diego, CA 92186-5608 (US) (For All Designated States Except US).
YUAN, Chong-Sheng [US/US]; (US) (For US Only).
DOU, Chao [CN/US]; (US) (For US Only)
Inventors: YUAN, Chong-Sheng; (US).
DOU, Chao; (US)
Agent: CHEN, Peng; Morrison & Foerster LLP, 12531 High Bluff Drive, Suite 100, San Diego, CA 92130-2040 (US).
LAUFER, Gabriele; Witte, Weller & Partner, Postfach 105462, 70047 Stuttgart (DE)
Priority Data:
10/916,563 11.08.2004 US
Title (EN) METHODS AND KITS FOR ASSAYING MYELOPEROXIDASE BY MEANS OF ENZYMATIC GLYCOLATE-GLYOXYLATE CYCLING REACTIONS
(FR) PROCEDES ET TROUSSES D'ESSAI DE LA MYELOPEROXIDASE UTILISANT DES REACTIONS ENZYMATIQUES DE CYCLAGE GLYCOLATE-GLYOXYLATE
Abstract: front page image
(EN)The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme (e.g. glycolaldehyde dehydrogenase) (1.2.1.21). The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.
(FR)L'invention porte sur un procédé d'essai de l'activité de la myéloperoxidase selon lequel un échantillon contenant de la myéloperoxidase ou susceptible d'en contenir est mis en contact avec un substrat comportant de la sérine, du peroxyde d'hydrogène et un halogénure. Si la myéloperoxidase est présente dans l'échantillon, la sérine est convertie en glycolaldéhyde qui est ensuite convertie en glycolate par une enzyme de conversion de la glycoaldéhyde (par exemple la glycoaldéhyde déshydrogénase) (1.2.1.21). Le procédé utilise un système de réaction de cyclage entre le glycolate et le glyoxylate pour produire un signal détectable correspondant à l'activité de la myéloperoxidase. L'invention porte également sur des trousses d'essai de la myéloperoxidase basées sur le même principe.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)