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Machine translation
1. (WO2006018270) CYTOCHROME P450 INDUCTION ASSAY
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/018270    International Application No.:    PCT/EP2005/008864
Publication Date: 23.02.2006 International Filing Date: 15.08.2005
IPC:
C12Q 1/68 (2006.01), C12N 15/53 (2006.01), C12N 5/10 (2006.01)
Applicants: ISTITUTO DI RICERCHE DI BIOLOGIA MOLECOLARE P ANGELETTI SPA [IT/IT]; Via Pontina KM 30.600, I-00040 Pomezia (Rome) (IT) (For All Designated States Except US).
CICUZZA, Sandra [IT/IT]; (IT) (For US Only).
LAUFER, Ralph [AT/IT]; (IT) (For US Only).
PAONESSA, Giacomo [IT/IT]; (IT) (For US Only)
Inventors: CICUZZA, Sandra; (IT).
LAUFER, Ralph; (IT).
PAONESSA, Giacomo; (IT)
Agent: MAN, Jocelyn; Merck Sharp & Dohme Limited, European Patent Department, Hertford Road, Hoddesdon, Hertfordshire EN11 9BU (GB)
Priority Data:
60/603,163 20.08.2004 US
Title (EN) CYTOCHROME P450 INDUCTION ASSAY
(FR) ESSAI D'INDUCTION DE CYTOCHROME P450
Abstract: front page image
(EN)A method for identifying compounds that can induce expression of cytochrome P450, in particular, expression of the CYP3A4 isoform, is described. The method provides a reporter gene operably linked to a composite promoter comprising in tandem one or more cis-acting elements, which are bound by activated pregnane X receptor (PXR), operably linked to a heterologous promoter. Analytes, which are inducers CYP3A4 expression via PXR activation, induce expression of the reporter gene.
(FR)L'invention concerne un procédé d'identification de composés pouvant induire l'expression de cytochrome P450, en particulier celle de l'isoforme CYP3A4. Le procédé consiste à fournir un gène rapporteur lié opérationnel à un promoteur composite qui présente en tandem un ou plusieurs éléments cis-agissants, liés par un récepteur prégnane X (PXR) activé, lié opérationnel à un promoteur hétérologue. Les analytes, inducteurs de l'expression de CYP3A4 par activation PXR, induisent l'expression du gène rapporteur.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)