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1. (WO2006015879) C-TERMINAL MODIFICATION OF POLYPEPTIDES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/015879    International Application No.:    PCT/EP2005/008809
Publication Date: 16.02.2006 International Filing Date: 12.08.2005
IPC:
C12N 9/76 (2006.01), C12Q 1/37 (2006.01), C12N 15/10 (2006.01)
Applicants: ROCHE DIAGNIOSTICS GMBH [DE/DE]; Sandhofer Strasse 116, 68305 Mannheim (DE) (DE only).
F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacharstrasse 124, CH-4070 Basel (CH) (AE, AG, AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CM, CN, CO, CR, CU, CY, CZ, DK, DM, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GW, HR, HU, ID, IE, IL, IN, IS, IT, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MC, MD, MG, MK, ML, MN, MR, MW, MX, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SI, SK, SL, SM, SN, SY, SZ, TD, TG, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, YU, ZA, ZM, ZW only).
HOESS, Eva [DE/DE]; (DE) (For US Only).
BORDUSA, Frank [DE/DE]; (DE) (For US Only).
HERRMANN, Rupert [DE/DE]; (DE) (For US Only).
JAKUBKE, Hans-Dieter [DE/DE]; (DE) (For US Only).
TISCHER, Wilhelm [AT/DE]; (DE) (For US Only)
Inventors: HOESS, Eva; (DE).
BORDUSA, Frank; (DE).
HERRMANN, Rupert; (DE).
JAKUBKE, Hans-Dieter; (DE).
TISCHER, Wilhelm; (DE)
Common
Representative:
ROCHE DIAGNIOSTICS GMBH; Naser, Werner, Patent Department (TR-E), Posftach 11 52, 82372 Penzberg (DE)
Priority Data:
04019237.9 13.08.2004 EP
Title (EN) C-TERMINAL MODIFICATION OF POLYPEPTIDES
(FR) MODIFICATION DU C-TERMINAL DE POLYPEPTIDES
Abstract: front page image
(EN)The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaal-Xaaz His, wherein Xaal is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.
(FR)Cette invention a pour objet une trypsine transformée, comprenant une substitution d’acide aminé en position K60 comme en position D189, et au moins une autre substitution d’acide aminé par de l’histidine en position N143 ou en position E151. Ce mutant de trypsine possède un site de reconnaissance préférable comprenant les acides aminés Xaal-Xaaz His, au sein duquel Xaal est L, Y ou F et Xaa2 est R ou K. Cette invention a également pour objet un polypeptide artificiel comprenant un peptide cible ainsi que le site de reconnaissance précité, et un procédé de production de peptides cibles à C-terminal modifié à l’aide de cette trypsine transformée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)