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1. (WO2005121334) LIPOLYTIC ENZYME ASSAY
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/121334    International Application No.:    PCT/DK2005/000375
Publication Date: 22.12.2005 International Filing Date: 07.06.2005
IPC:
C12N 9/20 (2006.01), C12N 15/55 (2006.01), C12Q 1/34 (2006.01), C12Q 1/44 (2006.01)
Applicants: NOVOZYMES A/S [DK/DK]; Krogshøjvej 36, DK-2880 Bagsværd (DK) (For All Designated States Except US).
BJØRNVAD, Mads Eskelund [DK/DK]; (DK) (For US Only).
ABEL, Gernot [AT/DK]; (DK) (For US Only)
Inventors: BJØRNVAD, Mads Eskelund; (DK).
ABEL, Gernot; (DK)
Common
Representative:
NOVOZYMES A/S; Krogshøjvej 36, DK-2880 Bagsværd (DK)
Priority Data:
PA 2004 00885 07.06.2004 DK
Title (EN) LIPOLYTIC ENZYME ASSAY
(FR) DOSAGE BIOLOGIQUE D'UNE ENZYME LIPOLYTIQUE
Abstract: front page image
(EN)The present invention relates to a method for measuring the activity of a lipolytic enzyme comprising measuring the pH of the gas phase of the enzymatic reaction between the lipolytic enzyme and a substrate, wherein the substrate comprises an ester bond between a C1-C10 carboxylic acid and an alcohol. Furthermore, it relates to a method for screening a library of polypeptide for a lipolytic enzyme of interest comprising testing the library in said method.
(FR)Cette invention concerne un procédé permettant de mesurer l'activité d'une enzyme lipolytique, lequel procédé consiste à mesurer le pH de la phase gazeuse de la réaction enzymatique entre l'enzyme lipolytique et un substrat, lequel substrat comprend une liaison ester entre un acide carboxylique C1-C10 et un alcool. en outre, cette invention concerne également un procédé permettant de cribler une banque de polypeptides pour une enzyme lipolytique présentant un intérêt, lequel procédé consiste à tester la banque susmentionnée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)