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1. (WO2005116213) DIRECTED EVOLUTION OF PROTEINS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2005/116213 International Application No.: PCT/US2005/013051
Publication Date: 08.12.2005 International Filing Date: 15.04.2005
IPC:
C12N 9/88 (2006.01) ,C12N 9/90 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90
Isomerases (5.)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US/US]; 17 Quincy Street Cambridge, MA 02138, US (AllExceptUS)
LIU, David; null (UsOnly)
LIU, Jane, M.; null (UsOnly)
BITTKER, Joshua, A.; null (UsOnly)
Inventors:
LIU, David; null
LIU, Jane, M.; null
BITTKER, Joshua, A.; null
Agent:
ENGELLENNER, Thomas, J. ; Nutter McClennen & Fish LLP World Trade Center West 155 Seaport Boulevard Boston, MA 02210-2604, US
Priority Data:
60/562,76115.04.2004US
Title (EN) DIRECTED EVOLUTION OF PROTEINS
(FR) EVOLUTION DIRIGEE DE PROTEINES
Abstract:
(EN) Disclosed is a method of altering a nucleic acid such as RNA or DNA. The method comprises fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a criterion. The methods of the invention can also be used to diversify proteins.
(FR) L'invention concerne un procédé qui permet de modifier un acide nucléique tel qu'un ARN ou un ADN. Selon le procédé de l'invention, on fragmente un brin d'acide nucléique parent afin de produire des fragments d'acide nucléique. On soumet au moins un sous-ensemble des fragments à une ligation afin d'obtenir des brins d'acide nucléique réarrangés. On identifie un brin choisi à partir des brins d'acide nucléique réarrangés sur la base d'un critère. Les procédés de l'invention peuvent également être utilisés pour diversifier les protéines.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)