WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2005113803) DETECTION OF AMPLICON CONTAMINATION DURING PCR EXHIBITING TWO DIFFERENT ANNEALING TEMPERATURES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/113803    International Application No.:    PCT/GB2005/001934
Publication Date: 01.12.2005 International Filing Date: 19.05.2005
IPC:
C12Q 1/68 (2006.01), B01L 3/00 (2006.01)
Applicants: AMPLION LIMITED [GB/GB]; 10 Helen Road, Oxford OX2 0DE (GB) (For All Designated States Except US).
NUNN, Miles [GB/GB]; (GB) (For US Only)
Inventors: NUNN, Miles; (GB)
Agent: ROQUES, Sarah, Elizabeth; J.A. Kemp & Co., 14 South Square, Gray's Inn, London WC1R 5JJ (GB)
Priority Data:
04090202.5 19.05.2004 EP
Title (EN) DETECTION OF AMPLICON CONTAMINATION DURING PCR EXHIBITING TWO DIFFERENT ANNEALING TEMPERATURES
(FR) DETECTION DE LA CONTAMINATION D'AMPLICONS LORS DE LA PCR PRESENTANT DEUX TEMPERATURES DE RECUIT DIFFERENTES
Abstract: front page image
(EN)A method to perform PCR reactions with one set of primers comprising sequence elements that are complementary to the target sequence and comprising sequence elements that server as tagging sequences. By conducting amplification reactions at different temperatures, the presence of contaminations arising from amplification products of previous reactions can be determined, improving reliability of the reaction and reducing the need for control reactions and reproduction of reactions.
(FR)L'invention concerne un procédé permettant d'effectuer des réactions PCR avec un ensemble d'amorces comprenant des éléments séquentiels qui sont complémentaires de la séquence cible ainsi que des éléments séquentiels qui servent de séquences de ciblage. En effectuant des réactions d'amplification à différentes températures, on peut déterminer la présence de contaminations découlant de produits d'amplification de réactions antérieures, ce qui améliore la fiabilité de la réaction et réduit la nécessité de réactions de contrôle ainsi que la reproduction de réactions.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)