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1. (WO2005108596) METHOD FOR PRODUCING CYCLIC PEPTIDES FROM IN VITRO PLANT CELL CULTURES

Pub. No.:    WO/2005/108596    International Application No.:    PCT/EP2005/004999
Publication Date: Nov 17, 2005 International Filing Date: May 9, 2005
IPC: C12P 21/00
Applicants: FRIEDRICH-ALEXANDER-UNIVERSITÄT ERLANGEN-NÜRNBERG
DÖRNENBURG, Heike
SEYDEL, Peter
BUCHHOLZ, Rainer
Inventors: DÖRNENBURG, Heike
SEYDEL, Peter
BUCHHOLZ, Rainer
Title: METHOD FOR PRODUCING CYCLIC PEPTIDES FROM IN VITRO PLANT CELL CULTURES
Abstract:
The present invention relates to a method for producing cyclic peptides, polypeptides or proteins in an in vitro culture system of plant origin Violaceae species, Cucurbitaceae species or Rubiaceae species comprising the following steps: (1) cultivating a plant cell or tissue culture derived from callus, organ, embryonic, suspension or single cell cultures, in one or more nutrient media, (2) inducing expression of one or more said cyclic peptides, polypeptides or proteins, (3) recovering one or more said cyclic peptides, polypeptides or proteins from said cell or tissue culture or said medium of said cell or tissue cultures, or both and wherein the cyclic peptides, polypeptides or proteins have a cyclic cysteine knot or its chemical or structural equivalent and at least three disulfide bonds.