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1. WO2005108588 - NOVEL HIV-BASED RECOMBINANT VIRAL CLONES AND USE THEREOF IN ANALYTICAL METHODS

Publication Number WO/2005/108588
Publication Date 17.11.2005
International Application No. PCT/ES2005/000250
International Filing Date 10.05.2005
Chapter 2 Demand Filed 12.12.2005
IPC
C12N 15/867 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
867Retroviral vectors
C12Q 1/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
CPC
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2740/16043
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
16011Human Immunodeficiency Virus, HIV
16041Use of virus, viral particle or viral elements as a vector
16043viral genome or elements thereof as genetic vector
Applicants
  • FUNDACIÓN PARA LA INVESTIGACIÓN Y LA PREVENCIÓN DEL SIDA EN ESPAÑA [ES]/[ES] (AllExceptUS)
  • INSTITUTO DE SALUD CARLOS III [ES]/[ES] (AllExceptUS)
  • ALCAMÍ PERTEJO, José [ES]/[ES] (UsOnly)
  • GARCÍA PEREZ, Javier [ES]/[ES] (UsOnly)
  • SÁNCHEZ PALOMINO, Sonsoles [ES]/[ES] (UsOnly)
  • GONZÁLEZ FERNÁNDEZ, Nuria [ES]/[ES] (UsOnly)
Inventors
  • ALCAMÍ PERTEJO, José
  • GARCÍA PEREZ, Javier
  • SÁNCHEZ PALOMINO, Sonsoles
  • GONZÁLEZ FERNÁNDEZ, Nuria
Agents
  • UNGRÍA LÓPEZ, Javier
Priority Data
P20040111610.05.2004ES
Publication Language Spanish (ES)
Filing Language Spanish (ES)
Designated States
Title
(EN) NOVEL HIV-BASED RECOMBINANT VIRAL CLONES AND USE THEREOF IN ANALYTICAL METHODS
(ES) NUEVOS CLONES VIRALES RECOMBINANTES BASADOS EN VIH Y SU UTILIZACIÓN EN MÉTODOS ANALÍTICOS
(FR) NOUVEAUX CLONES VIRAUX RECOMBINES BASES SUR LE VIH ET LEUR UTILISATION DANS DES METHODES ANALYTIQUES
Abstract
(EN)
The invention relates to HIV-based recombinant viral clones having the general structure shown in the accompanying figure, which are the result of the following genetic manipulations: deletion of HIV fragments (e.g. the Nef gene) without loss of infective capacity; insertion of a gene that is not expressed in human cells; insertion of the LacZ gene; and introduction of restriction sites, in order to extract DNA fragments from the matrix provirus and substitute same with genes from the patients to be assessed. In addition, the invention relates to the application of said clones in analytical methods that are associated with AIDS.
(ES)
La presente invención se refiere a clones vírales recombinantes basados en VIH que poseen la estructura general representada en la figura 8 y son el resultado de las siguientes manipulaciones genéticas: -deleción de fragmentos de VIH (por ejemplo, gen Nef) sin perder capacidad infectiva, -inserción de un gen no expresado en células humanas, -inserción del gen LacZ, -introducción de sitios de restricción para extraer fragmentos de ADN del provirus matriz y sustituirlos por genes de pacientes a valorar. Asimismo, la invención se refiere a la aplicación de dichos clones en métodos analíticos relacionados con el SIDA.
(FR)
La présente invention concerne des clones viraux recombinés basés sur le VIH lesquels possèdent la structure générale représentée par la figure et sont le résultat des manipulations génétiques suivantes: délétion de fragments de VIH (par exemple, gène Nef) sans perte de capacité d'infection, insertion d'un gène non exprimé dans des cellules humaines, insertion du gène Lacz, introduction de sites de restriction pour extraire des fragments d'ADN du provirus matriciel et pour les remplacer par des gènes de patients à évaluer. De même, l'invention concerne l'application desdits clones à des méthodes analytiques concernant le SIDA.
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