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1. (WO2005100542) METHOD FOR PURIFYING PLASMID DNA
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2005/100542 International Application No.: PCT/EP2005/005213
Publication Date: 27.10.2005 International Filing Date: 19.04.2005
IPC:
C12N 1/06 (2006.01) ,C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
06
Lysis of micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
BLANCHE, Francis [FR/FR]; FR (UsOnly)
COUDER, Michel [FR/FR]; FR (UsOnly)
MAESTRALI, Nicolas [FR/FR]; FR (UsOnly)
GUILLEMIN, Thierry [FR/FR]; FR (UsOnly)
GAILLAC, David [FR/FR]; FR (UsOnly)
CENTELION [FR/FR]; 72-82 rue Leon Geffroy F-94400 Vitry-sur-Seine, FR (AllExceptUS)
Inventors:
BLANCHE, Francis; FR
COUDER, Michel; FR
MAESTRALI, Nicolas; FR
GUILLEMIN, Thierry; FR
GAILLAC, David; FR
Agent:
LECCA, Patricia; Centelion 72-82 rue Leon Geffroy F-94400 Vitry-sur-Seine, FR
Priority Data:
60/563,00819.04.2004US
PCT/EP2004/01143717.09.2004EP
Title (EN) METHOD FOR PURIFYING PLASMID DNA
(FR) PROCEDE DESTINE A PURIFIER DE L'ADN PLASMIDIQUE
Abstract:
(EN) This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants.
(FR) L'invention concerne un procédé de lyse alcaline continue d'une suspension bactérienne en vue de cultiver de l'ADNp. Elle concerne également des étapes de purification supplémentaires facultatives, notamment la filtration de lysat, la chromatographie échangeuse d'anions, la chromatographie par affinité de triplex et la chromatographie hydrophobe. Ces étapes de purification facultatives peuvent être combinées à la lyse continue en vue de produire un produit d'ADNp hautement purifié sensiblement dépourvu d'ADNg, d'ARN, de protéines, d'endotoxines, et d'autres contaminants.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)