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1. WO2005098029 - METHODS FOR QUANTIFICATION OF MICRORNAS AND SMALL INTERFERING RNAS

Publication Number WO/2005/098029
Publication Date 20.10.2005
International Application No. PCT/DK2005/000239
International Filing Date 07.04.2005
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6851
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6851Quantitative amplification
C12Q 1/6853
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6853using modified primers or templates
Applicants
  • EXIQON A/S [DK]/[DK] (AllExceptUS)
  • JACOBSEN, Nana [DK]/[DK] (UsOnly)
  • KONGSBAK, Lars [DK]/[DK] (UsOnly)
  • KAUPPINEN, Sakari [FI]/[DK] (UsOnly)
  • ECHWALD, Søren, Morgenthaler [DK]/[DK] (UsOnly)
  • MOURITZEN, Peter [DK]/[DK] (UsOnly)
  • NIELSEN, Peter, Stein [DK]/[DK] (UsOnly)
  • NØRHOLM, Mikkel [DK]/[DK] (UsOnly)
Inventors
  • JACOBSEN, Nana
  • KONGSBAK, Lars
  • KAUPPINEN, Sakari
  • ECHWALD, Søren, Morgenthaler
  • MOURITZEN, Peter
  • NIELSEN, Peter, Stein
  • NØRHOLM, Mikkel
Agents
  • INSPICOS A/S
Priority Data
PA 2004 0057807.04.2004DK
PA 2004 0114623.07.2004DK
PA 2004 0121811.08.2004DK
PA 2004 0158715.10.2004DK
PA 2005 0014028.01.2005DK
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR QUANTIFICATION OF MICRORNAS AND SMALL INTERFERING RNAS
(FR) NOUVELLES METHODES PERMETTANT DE QUANTIFIER DES MICRO ARN ET PETITS ARN INTERFERANTS
Abstract
(EN)
The invention relates to ribonucleic acids, probes and methods for detection, quantification as well as monitoring the expression of mature microRNAs and small interfering RNAs (siRNAs). The invention furthermore relates to methods for monitoring the expression of other non-coding RNAs, mRNA splice variants, as well as detecting and quantifying RNA editing, allelic variants of single transcripts, mutations, deletions, or duplications of particular exons in transcripts, e.g., alterations associated with human disease such as cancer. The invention furthermore relates to methods for detection, quantification as well as monitoring the expression of deoxy nucleic acids.
(FR)
L'invention concerne des acides ribonucléiques, des sondes et des méthodes permettant de détecter, de quantifier et de surveiller l'expression de micro-ARN matures et de petits ARN interférants (siARN). L'invention concerne également des méthodes permettant de surveiller l'expression d'autres ARN non codants et de variantes d'épissure d'ARNm, de détecter et de quantifier une édition d'ARN, des variantes alléliques de transcriptions uniques, des mutations, des suppressions ou des duplications d'exons particuliers dans des transcriptions, par exemple, des altérations associées à une maladie humaine telle que le cancer. L'invention concerne enfin des méthodes permettant de détecter, de quantifier et de surveiller l'expression d'acides déoxy ribo nucléiques.
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