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1. WO2005097969 - APPARATUS AND METHODS FOR SIMULTANEOUS OPERATION OF MINIATURIZED REACTORS

Publication Number WO/2005/097969
Publication Date 20.10.2005
International Application No. PCT/US2005/011295
International Filing Date 01.04.2005
IPC
C12M 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
CPC
B01F 13/0059
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13Other mixers; Mixing plant, including combinations of ; mixers, e.g. of; dissimilar mixers
0059Micromixers
B01F 13/0827
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
13Other mixers; Mixing plant, including combinations of ; mixers, e.g. of; dissimilar mixers
08Magnetic mixers ; ; Mixers having magnetically driven stirrers
0827using supported or suspended stirring elements
B01J 2219/00283
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00279Features relating to reactor vessels
00281Individual reactor vessels
00283Reactor vessels with top opening
B01J 2219/00317
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00279Features relating to reactor vessels
00306Reactor vessels in a multiple arrangement
00313the reactor vessels being formed by arrays of wells in blocks
00315Microtiter plates
00317Microwell devices, i.e. having large numbers of wells
B01J 2219/00479
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00479Means for mixing reactants or products in the reaction vessels
B01J 2219/00481
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
2219Chemical, physical or physico-chemical processes in general; Their relevant apparatus
00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
00277Apparatus
00479Means for mixing reactants or products in the reaction vessels
00481by the use of moving stirrers within the reaction vessels
Applicants
  • MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US]/[US] (AllExceptUS)
  • BOCCAZZI, Paolo [IT]/[US] (UsOnly)
  • CHEN, Angela, Y. [US]/[US] (UsOnly)
  • JENSEN, Klavs, F. [US]/[US] (UsOnly)
  • SZITA, Nicolas [CH]/[US] (UsOnly)
  • ZANZOTTO, Andrea [CA]/[US] (UsOnly)
  • ZHANG, Zhiyu [CN]/[US] (UsOnly)
Inventors
  • BOCCAZZI, Paolo
  • CHEN, Angela, Y.
  • JENSEN, Klavs, F.
  • SZITA, Nicolas
  • ZANZOTTO, Andrea
  • ZHANG, Zhiyu
Agents
  • GERBER, Monica, R.
Priority Data
10/816,04601.04.2004US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) APPARATUS AND METHODS FOR SIMULTANEOUS OPERATION OF MINIATURIZED REACTORS
(FR) DISPOSITIFS ET PROCEDES SERVANT A EFFECTUER L'OPERATION SIMULTANEE DE REACTEURS MINIATURISES
Abstract
(EN)
The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel (2) for culturing cells and means for providing oxygen (6) to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane (6) and optionally a variety of sensing devices (12). The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.
(FR)
L'invention concerne une variété de bioréacteurs miniaturisés (microfermenteurs) et d'ensembles de ces bioréacteurs miniaturisés conçus pour être mis en application dans la culture de cellules. Ces microfermenteurs comprennent une cuve (2) servant à cultiver les cellules et des moyens servant à alimenter l'intérieur de la cuve en oxygène (6) à une concentration suffisante pour permettre la croissance cellulaire, par exemple, la croissance de cellules bactériennes. Selon les différents modes de réalisation, la cuve du microfermenteur peut présenter différents volumes intérieurs inférieurs à approximativement 1 ml. Ces microfermenteurs peuvent comporter une membrane d'aération (6) et éventuellement une variété de dispositifs de détection (12). L'invention concerne également une chambre conçue pour contenir les microfermenteurs et des ensembles de microfermenteurs, ainsi que pour exercer un contrôle sur l'environnement. Elle concerne des microréacteurs possédant une variété de conception différente, dont certains incorporent une capacité d'agitation active et/ou d'opération en lots ou en mode d'alimentation en lots. Elle concerne, de plus, un dispositif et des procédés servant à effectuer l'opération simultanée d'une pluralité de microréacteurs, tout en contrôlant les microréacteurs individuels pendant une session. Elle concerne également des procédés servant à exécuter une analyse d'expression génique sur des cellules cultivées dans ces microréacteurs.
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