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1. WO2005095576 - CONTROLLED TEMPERATURE WATER (OR OTHER FLUID)-JACKET CO2 MICROSCOPE STAGE INCUBATOR

Publication Number WO/2005/095576
Publication Date 13.10.2005
International Application No. PCT/IT2005/000161
International Filing Date 24.03.2005
IPC
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12M 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/38 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
36including condition or time responsive control, e.g. automatically controlled fermentors
38Temperature-responsive control
G02B 21/34 2006.01
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
34Microscope slides, e.g. mounting specimens on microscope slides
CPC
C12M 23/54
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
54hand portable
C12M 41/12
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
41Means for regulation, monitoring, measurement or control, e.g. flow regulation
12of temperature
C12M 41/14
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
41Means for regulation, monitoring, measurement or control, e.g. flow regulation
12of temperature
14Incubators; Climatic chambers
C12M 41/22
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
41Means for regulation, monitoring, measurement or control, e.g. flow regulation
12of temperature
18Heat exchange systems, e.g. heat jackets or outer envelopes
22in contact with the bioreactor walls
C12M 41/36
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
41Means for regulation, monitoring, measurement or control, e.g. flow regulation
30of concentration
36of biomass, e.g. colony counters or by turbidity measurements
C12M 41/46
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
41Means for regulation, monitoring, measurement or control, e.g. flow regulation
46of cellular or enzymatic activity or functionality, e.g. cell viability
Applicants
  • HIGH TECH CONSULTING S.R.L. [IT]/[IT] (AllExceptUS)
  • LANZARO, Luca [IT]/[IT]
  • CASERTA, Sergio
  • GUIDO, Stefano
  • SABETTA, Luigi
  • SIBILLO, Vincenzo
  • SIMEONE, Marino
Inventors
  • LANZARO, Luca
  • CASERTA, Sergio
  • GUIDO, Stefano
  • SABETTA, Luigi
  • SIBILLO, Vincenzo
  • SIMEONE, Marino
Common Representative
  • LANZARO, Luca
Priority Data
NA2004A00001602.04.2004IT
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) CONTROLLED TEMPERATURE WATER (OR OTHER FLUID)-JACKET CO2 MICROSCOPE STAGE INCUBATOR
(FR) INCUBATEUR A ETAGE MICROSCOPE POUR UNE ENVELOPPE D'EAU (OU TOUT AUTRE FLUIDE) CONTENANT DU CO2 A TEMPERATURE COMMANDEE
Abstract
(EN)
Controlled temperature water (or other fluid)-jacket CO2 Microscope Stage Incubator developed by “High Tech Consulting s.r.l.” is designed to maintain all the required environmental conditions for cell cultures (or other biological species) right on the microscope stage, thus allowing prolonged observations of cell (or biological) events. It consist of a chamber made of steel or aluminium or other materials, and it has dimensions so that it can fit right on the microscope stage. It enables to stably maintain the environmental conditions of temperature and/or humidity level and/or CO2 (or other gases) level required to study biological phenomena. Desired temperature conditions are provided by inner water (or different fluid) circulation and are guaranteed by the joint action of a PID software controller, with the thermocouple directly inserted inside the incubator. Humidity and CO2 levels are maintained by feeding a preconditioned air stream regulated by flow rate controllers. Holes are drilled into the chamber bottom to host samples for observation.
(FR)
L'invention concerne un incubateur à étage microscope pour une enveloppe d'eau contenant du CO2 ou tout autre fluide à température commandée, développé par High Tech Consulting s.r.l. , ledit incubateur étant conçu pour maintenir toutes les conditions environnementales pour la mise en culture des cellules (ou toute autre espèce biologique) sur l'étage du microscope, ce qui permet la réalisation d'observations prolongées d'événements cellulaires ou biologiques. Ledit incubateur comprend une chambre fabriquée en acier ou en aluminium ou tout autre matériau, et il présente des dimensions permettant de le placer verticalement sur l'étage microscope. Cela permet de maintenir de manière stable les conditions environnementales de la température et/ou de la teneur en humidité et/ou en CO2 (ou tout autre gaz) nécessaire à l'étude des phénomènes biologiques. Des conditions de température souhaitée sont fournies par la circulation de l'eau interne (ou d'autre fluide) et sont garanties grâce à l'action conjointe d'un dispositif de commande de logiciel PID, le thermocouple étant directement inséré à l'intérieur de l'incubateur. Les teneurs en humidité et en CO2 sont maintenues grâce à l'alimentation d'un flux d'air préconditionné regulé par des dispositifs de régulation de débits. Des orifices sont percés dans le fond de la chambre vers des échantillons hôtes, ce qui permet de les observer.
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