WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
PATENTSCOPE will be unavailable a few hours for maintenance reason on Saturday 18.08.2018 at 9:00 AM CEST
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2005068630) DOUBLE-STRANDED RNA FOR INTERFERENCE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2005/068630 International Application No.: PCT/JP2004/018847
Publication Date: 28.07.2005 International Filing Date: 16.12.2004
IPC:
A61K 48/00 (2006.01) ,C12N 5/10 (2006.01) ,C12N 15/11 (2006.01) ,C12N 15/63 (2006.01)
Applicants: TAIRA, Kazunari[JP/JP]; JP (UsOnly)
SUZUKI, Tsutomu[JP/JP]; JP (UsOnly)
MIYAGISHI, Makoto[JP/JP]; JP (UsOnly)
MATSUMOTO, Sahohime[JP/JP]; JP (UsOnly)
KATOU, Takayuki[JP/JP]; JP (UsOnly)
National Institute of Advanced Industrial Science and Technology[JP/JP]; 3-1, Kasumigaseki 1-chome, Chiyoda-ku, Tokyo 1008921, JP (AllExceptUS)
Inventors: TAIRA, Kazunari; JP
SUZUKI, Tsutomu; JP
MIYAGISHI, Makoto; JP
MATSUMOTO, Sahohime; JP
KATOU, Takayuki; JP
Agent: OKUYAMA, Shoichi; 8th Floor, Akasaka NOA Bldg., 2-12, Akasaka 3-chome, Minato-ku Tokyo 1070052, JP
Priority Data:
2003-41752416.12.2003JP
Title (EN) DOUBLE-STRANDED RNA FOR INTERFERENCE
(FR) ARN BICATENAIRE INTERFERANT
(JA) 干渉用二重鎖RNA
Abstract:
(EN) For RNAi, use is made of a double-stranded RNA for interference which comprises an antisense RNA complementary to one region in the mRNA of a target gene and a sense RNA homologous with the above region in the mRNA of the above target gene, characterized in that the antisense RNA and the sense RNA as described above together form a double-strand over consecutive 25 bases, 26 bases, 27 bases or 28 or more bases. Also, use can be made of a double-stranded RNA having base substitution or base insertion. By using this double-stranded RNA for interference as described above, RNAi with relieved cytotoxicity can be induced at a high efficiency.
(FR) L'invention concerne l'ARNi avec l'utilisation d'un ARN bicaténaire interférant comportant un ARN anti-sens complémentaire d'une région de l'ARNm d'un gène cible et un ARN sens homologue de ladite région de l'ARNm dudit gène cible. Selon l'invention, ledit ARN anti-sens et ledit ARN sens forment ensemble un double brin présentant consécutivement 25 bases, 26 bases, 27 bases 28 bases ou davantage de bases. De plus, un ARN bicaténaire présentant une substitution de bases ou une insertion de bases peut être utilisé. On peut ainsi induire l'ARNi à toxicité réduite de manière efficace, en utilisant ledit ARN bicaténaire interférant.
(JA)  標的遺伝子のmRNAにおけるいずれかの領域と相補的なアンチセンスRNAと、前記標的遺伝子のmRNAにおける前記領域と相同なセンスRNAとを含む干渉用二重鎖RNAであって、前記アンチセンスRNAと前記センスRNAとが連続した25塩基、26塩基、27塩基、又は28以上の塩基にわたって二重鎖を形成していることを特徴とする干渉用二重鎖RNAをRNAiに用いる。塩基置換及び塩基の挿入を含む二重鎖RNAを用いることもできる。本発明の干渉用二重鎖RNAにより、細胞毒性が軽減されたRNAiを高い効率で誘導することができる。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)