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1. WO2005064019 - UNIVERSAL AMPLIFICATION OF FRAGMENTED RNA

Publication Number WO/2005/064019
Publication Date 14.07.2005
International Application No. PCT/US2004/043163
International Filing Date 21.12.2004
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/1096
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1096cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Applicants
  • GENOMIC HEALTH, INC. [US]/[US] (AllExceptUS)
  • KIEFER, Michael, C. [US]/[US] (UsOnly)
  • HOYT, Kenneth, W. (UsOnly)
Inventors
  • KIEFER, Michael, C.
  • HOYT, Kenneth, W.
Agents
  • DREGER, Ginger, R.
Priority Data
60/532,68423.12.2003US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) UNIVERSAL AMPLIFICATION OF FRAGMENTED RNA
(FR) AMPLIFICATION UNIVERSELLE D'ARN FRAGMENTE
Abstract
(EN)
The invention relates to methods of using fragmented RNA, such as RNA obtained from archived fixed paraffin-embedded tissue material (FPET RNA) or other clinically biopsied tissue specimens for universal gene expression profiling.
(FR)
L'invention concerne des méthodes qui permettent d'utiliser un ARN fragmenté, tel qu'un ARN obtenu à partir d'un matériau de tissu fixé et archivé inclus en paraffine (ARN FPET) ou d'autres prélèvements tissulaires ponctionnés cliniquement pour un profilage d'expression d'un gène universel.
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