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1. (WO2005062820) MULTI PLASMID SYSTEM FOR THE PRODUCTION OF INFLUENZA VIRUS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2005/062820 International Application No.: PCT/US2004/042669
Publication Date: 14.07.2005 International Filing Date: 22.12.2004
IPC:
C12N 15/63 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
Applicants:
DUKE, Greg [US/US]; US (UsOnly)
KEMBLE, George [US/US]; US (UsOnly)
MEDIMMUNE VACCINES, INC [US/US]; c/o MedImmune Inc. One MedImmune Way Gaithersburg, Maryland 20878, US (AllExceptUS)
Inventors:
DUKE, Greg; US
KEMBLE, George; US
Agent:
ALBAN, Patrick Scott; MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878, US
Priority Data:
60/532,16423.12.2003US
Title (EN) MULTI PLASMID SYSTEM FOR THE PRODUCTION OF INFLUENZA VIRUS
(FR) SYSTEME A PLASMIDES MULTIPLES POUR LA PRODUCTION DU VIRUS DE LA GRIPPE
Abstract:
(EN) Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.
(FR) La présente invention a trait à des vecteurs et des procédés pour la production de virus de la grippe appropriés en tant que vaccins recombinants de la grippe en culture cellulaire. L'invention a trait à des vecteurs d'expression bidirectionnelle destinés à être utilisés dans un système à plasmides multiples d'expression du virus de la grippe. L'invention a trait en outre à des procédés de production de virus de la grippe avec une capacité accrue de réplication dans des oeufs embryonnés et/ou de cellules de poulets (par exemple, des cellules Vero et/ou MDCK) et également à des virus de la grippe avec des caractéristiques de réplication améliorées. En outre, la présente invention a trait à un procédé amélioré de sauvetage, dans lequel des cellules animales (par exemple, des cellules souches SF Vero) sont soumises à une électroporation avec des plasmides et des vecteurs de l'invention.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)