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1. WO2005061731 - METHOD AND SYSTEM FOR UNBIASED GENOME AMPLIFICATION USING GENETIC ALGORITHMS TO SELECT PRIMERS FOR GENOMIC DNA AMPLIFICATION

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Publication Number WO/2005/061731
Publication Date 07.07.2005
International Application No. PCT/SG2004/000344
International Filing Date 18.10.2004
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G06F 15/18 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
FELECTRIC DIGITAL DATA PROCESSING
15Digital computers in general; Data processing equipment in general
18in which a program is changed according to experience gained by the computer itself during a complete run; Learning machines
G06N 3/12 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
NCOMPUTER SYSTEMS BASED ON SPECIFIC COMPUTATIONAL MODELS
3Computer systems based on biological models
12using genetic models
CPC
G16B 25/00
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
25ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
G16B 40/00
GPHYSICS
16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
40ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
Applicants
  • NANYANG POLYTECHNIC [SG]/[SG] (AllExceptUS)
  • KON, Oi, Lian [CA]/[SG] (UsOnly)
  • PNG EAK HOCK, Adrian [SG]/[SG] (UsOnly)
  • CHOO, Keng, Wah [MY]/[SG] (UsOnly)
Inventors
  • KON, Oi, Lian
  • PNG EAK HOCK, Adrian
  • CHOO, Keng, Wah
Agents
  • LAWRENCE Y D HO & ASSOCIATES PTE LTD
Priority Data
200307729-424.12.2003SG
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND SYSTEM FOR UNBIASED GENOME AMPLIFICATION USING GENETIC ALGORITHMS TO SELECT PRIMERS FOR GENOMIC DNA AMPLIFICATION
(FR) PROCEDE ET SYSTEME D'AMPLIFICATION GENOMIQUE NON POLARISEE UTILISANT DES ALGORITHMES GENETIQUES AFIN DE SELECTIONNER DES AMORCES POUR L'AMPLIFICATION D'ADN GENOMIQUE
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Abstract
(EN)
The present invention provides systems and methods for determining optimal primers to be used for unbiased genome amplification. The methods for determining the optimal primers employ computer-based algorithms. In one embodiment, a genetic algorithms-based method for selecting primers for genomic DNA amplification is provided. In addition, the present invention provides a system for executing genetic algorithms-based methods for selecting primers for genomic DNA amplification. Furthermore, the present invention provides a number of hexamers that can be used for unbiased amplification of human genomic DNA.
(FR)
L'invention a pour objet des systèmes et des procédés de détermination d'amorces optimales que l'on peut utiliser pour l'amplification génomique non polarisée. Les procédés de détermination des amorces optimales utilisent des algorithmes informatiques. Dans un mode de réalisation, le procédé utilisant des algorithmes génétiques permettant de sélectionner des amorces pour l'amplification d'ADN génomique fait également l'objet de cette invention. Par ailleurs, l'invention a pour objet un système d'exécution de procédé utilisant des algorithmes génétiques permettant de sélectionner des amorces pour l'amplification d'ADN génomique. L'invention a finalement pour objet un certain nombre d'hexamères que l'on peut utiliser pour l'amplification non polarisée d'un ADN génomique humain.
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