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1. (WO2005036123) METHODS FOR SUBSTANTIALLY SIMULTANEOUS EVALUATION OF A SAMPLE CONTAINING A CELLULAR TARGET AND A SOLUBLE ANALYTE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/036123    International Application No.:    PCT/US2004/024235
Publication Date: 21.04.2005 International Filing Date: 28.07.2004
Chapter 2 Demand Filed:    22.04.2005    
IPC:
G01N 33/546 (2006.01), G01N 33/564 (2006.01), G01N 33/574 (2006.01), A61K 39/395 (2006.01)
Applicants: BECKMAN COULTER, INC. [US/US]; 4300 N. Harbor Blvd., Fullerton, CA 92834 (US) (For All Designated States Except US).
MILLS, Rhonda, A. [US/US]; (US) (For US Only).
QUINTANA, Jorge, A. [US/US]; (US) (For US Only).
MAPLES, John, A. [US/US]; (US) (For US Only).
SCIBELLI, Paul, M. [US/US]; (US) (For US Only).
HASHIMOTO, Wataru [JP/JP]; (JP) (For US Only)
Inventors: MILLS, Rhonda, A.; (US).
QUINTANA, Jorge, A.; (US).
MAPLES, John, A.; (US).
SCIBELLI, Paul, M.; (US).
HASHIMOTO, Wataru; (JP)
Agent: HAILE, Lisa, A.; Gray Cary Ware & Freidenrich LLP, 4365 Executive Drive, Suite 1100, San Diego, CA 92121-2133 (US)
Priority Data:
10/672,477 26.09.2003 US
Title (EN) METHODS FOR SUBSTANTIALLY SIMULTANEOUS EVALUATION OF A SAMPLE CONTAINING A CELLULAR TARGET AND A SOLUBLE ANALYTE
(FR) PROCEDES POUR L'EVALUATION PRATIQUEMENT SIMULTANEE D'UN ECHANTILLON CONTENANT UNE CIBLE CELLULAIRE ET UN ANALYTE SOLUBLE
Abstract: front page image
(EN)Methods are provided for monitoring treatment of a subject using heparin therapy or a ligand, such as an CD20 or CD52 antibody, that binds to a cellular marker and to which the subject may develop masking reactions or auto-antibodies that inhibit evaluation of treatment. The methods involve adding to a single container a sample containing cells that express the cellular target with (i) a soluble ligand that binds the cellular target, (ii) a soluble ligand that binds the soluble analyte or a competing soluble analyte; and (iii) a capture medium that binds directly to the soluble analyte, indirectly to the soluble analyte, or to the soluble ligand that binds to the soluble analyte. Complexes formed in the container by interaction of these components are substantially simultaneously analyzed and quantitated without physically separating the complexes. Kits for performing the assays are also provided.
(FR)L'invention concerne des procédés servant à surveiller le traitement d'un sujet par héparinothérapie ou au moyen d'un ligand, un anticorps CD20 ou CD52 par exemple, qui se lie à un marqueur cellulaire et envers lequel le sujet peut développer des réactions de masquage ou des auto-anticorps inhibant l'évaluation du traitement. Les procédés selon l'invention consistent à ajouter, dans un récipient unique contenant un échantillon qui contient des cellules exprimant la cible cellulaire, (i) un ligand soluble qui se lie à la cible cellulaire, (ii) un ligand soluble qui se lie à l'analyte soluble ou à un analyte soluble concurrent et (iii) un milieu de capture qui se lie directement à l'analyte soluble, qui se lie indirectement à l'analyte soluble ou qui se lie au ligand soluble qui se lie à l'analyte soluble. Des complexes formés dans le récipient par interaction de ces composants sont analysés et quantifiés pratiquement simultanément sans séparation physique des complexes. L'invention concerne également des nécessaires pour effectuer les dosages biologiques.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)