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1. (WO2005035718) BIDERECTIONAL PROMOTERS FOR SMALL RNA EXPRESSION
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2005/035718 International Application No.: PCT/US2004/032158
Publication Date: 21.04.2005 International Filing Date: 02.10.2004
IPC:
A01N 43/04 (2006.01) ,C07H 21/04 (2006.01) ,C12N 15/00 (2006.01) ,C12Q 1/68 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
43
Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
02
having rings with one or more oxygen or sulfur atoms as the only ring hetero atom
04
with one hetero atom
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants: LOU, Ke[CN/US]; US (UsOnly)
WELGEN, INC.[US/US]; 25 Winthrop Street Worcester, MA 01604, US (AllExceptUS)
FRICK, G., Peter[US/US]; US (UsOnly)
DU, Ling[CH/US]; US (UsOnly)
Inventors: LOU, Ke; US
Agent: WANG, Gary, F.; 201 South Lake Avenue Suite 302 Pasadena, CA 91101, US
Priority Data:
60/508,82103.10.2003US
null02.10.2004US
Title (EN) BIDERECTIONAL PROMOTERS FOR SMALL RNA EXPRESSION
(FR) PROMOTEURS BIDIRECTIONNELS POUR L'EXPRESSION DE PETITES SEQUENCES D'ARN
Abstract:
(EN) The invention provides bidirectional promoters for expressing two or more short RNA sequences from a single promoter. A particular embodiment of the bidirectional promoters of the invention include: 1) a Pol III promoter that contains a TATA box, a PSE and a DSE; and 2) a Pol III promoter that includes a PSE and a TATA box fused to the 5’ end of said DSE in reverse orientation. Vector embodiments are also disclosed comprising the novel bidirectional promoters of the invention, as well as methods of making and using these promoters.
(FR) L'invention concerne des promoteurs bidirectionnels pour l'expression de deux ou plusieurs courtes séquences d'ARN à partir d'un seul promoteur. Un mode de réalisation particulier des promoteurs bidirectionnels de l'invention comprend : 1) un promoteur pol III contenant une boîte TATA, un PSE ainsi qu'un DSE et 2) un promoteur pol III contenant un PSE et une boîte TATA fusionnés à l'extrémité 5' dudit DSE en orientation inverse. L'invention concerne également des modes de réalisation de vecteurs, comprenant les nouveaux promoteurs bidirectionnels de l'invention, ainsi que des procédés de production et d'utilisation desdits promoteurs.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)