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Machine translation
1. (WO2005033271) METHODS OF RAPID DETECTION AND IDENTIFICATION OF BIOAGENTS USING microRNA
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/033271    International Application No.:    PCT/US2004/028869
Publication Date: 14.04.2005 International Filing Date: 07.09.2004
IPC:
C12Q 1/68 (2006.01), C12P 19/34 (2006.01)
Applicants: ISIS PHARMACEUTICALS, INC. [US/US]; 2292 Faraday Avenue, Carlsbad, CA 92008 (US) (For All Designated States Except US).
GRIFFEY, Richard, H. [US/US]; (US) (For US Only).
ECKER, David, J. [US/US]; (US) (For US Only)
Inventors: GRIFFEY, Richard, H.; (US).
ECKER, David, J.; (US)
Agent: LEGAARD, Paul, K.; Cozen O'Connor, 1900 Market Street, Philadelphia, PA 19103 (US)
Priority Data:
60/500,722 04.09.2003 US
60/505,147 17.09.2003 US
Title (EN) METHODS OF RAPID DETECTION AND IDENTIFICATION OF BIOAGENTS USING microRNA
(FR) PROCEDES DE DETECTION ET D'IDENTIFICATION RAPIDES D'AGENTS BIOLOGIQUES A L'AIDE DE MICRO-ARN
Abstract: front page image
(EN)Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a 'base composition signature' (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.
(FR)L'invention concerne des procédés de détection et d'identification d'agents biologiques inconnus tels que des bactéries, des virus et similaires, par combinaison de l'amplification de micro-ARN contenant des acides nucléiques et de la détermination du poids moléculaire dudit micro-ARN, à l'aide d'amorces s'hybridant à des zones de séquences inchangées du micro-ARN contenant des acides nucléiques, dérivant d'un agent biologique, et encadrant des zones de séquences variables identifiant l'agent biologique de façon unique. On obtient ainsi une 'signature de composition de base' (BCS) ou un poids moléculaire comparée à une base de données de signatures de compositions de base ou de poids moléculaire servant à l'identification de l'espèce de l'agent biologique.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)