Processing

Please wait...

Settings

Settings

Goto Application

1. WO2005024002 - RECOMBINANT PRODUCTION OF ANTIMICROBIAL AGENTS

Publication Number WO/2005/024002
Publication Date 17.03.2005
International Application No. PCT/DK2004/000605
International Filing Date 13.09.2004
IPC
A23K 1/16 2006.1
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
KFEEDING-STUFFS SPECIALLY ADAPTED FOR ANIMALS; METHODS SPECIALLY ADAPTED FOR PRODUCTION THEREOF
1Animal feeding-stuffs
16supplemented with accessory food factors; Salt blocks
A23K 1/165 2006.1
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
KFEEDING-STUFFS SPECIALLY ADAPTED FOR ANIMALS; METHODS SPECIALLY ADAPTED FOR PRODUCTION THEREOF
1Animal feeding-stuffs
16supplemented with accessory food factors; Salt blocks
165with steroids, hormones, or enzymes
C07K 14/47 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
C12N 15/62 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 15/79 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
C12Q 1/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
CPC
A01K 2217/05
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2217Genetically modified animals
05Animals comprising random inserted nucleic acids (transgenic)
A23K 20/147
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
KFODDER
20Accessory food factors for animal feeding-stuffs
10Organic substances
142Amino acids; Derivatives thereof
147Polymeric derivatives, e.g. peptides or proteins
A23K 20/189
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
KFODDER
20Accessory food factors for animal feeding-stuffs
10Organic substances
189Enzymes
C07K 14/4723
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
46from vertebrates
47from mammals
4701not used
4723Cationic antimicrobial peptides, e.g. defensins
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C07K 2319/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
02containing a signal sequence
Applicants
  • NOVOZYMES A/S [DK]/[DK] (AllExceptUS)
  • JENSEN, Ejner, Bech [DK]/[DK] (UsOnly)
  • HØGENHAUG, Hans-Henrik, Kristensen [DK]/[DK] (UsOnly)
  • HANSEN, Peter, Kamp [DK]/[DK] (UsOnly)
  • PEDERSEN, Poul, Erik [DK]/[DK] (UsOnly)
  • MYGIND, Per, Holse [DK]/[DK] (UsOnly)
Inventors
  • JENSEN, Ejner, Bech
  • HØGENHAUG, Hans-Henrik, Kristensen
  • HANSEN, Peter, Kamp
  • PEDERSEN, Poul, Erik
  • MYGIND, Per, Holse
Common Representative
  • NOVOZYMES A/S
Priority Data
PA 2003 0131011.09.2003DK
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) RECOMBINANT PRODUCTION OF ANTIMICROBIAL AGENTS
(FR) PRODUCTION RECOMBINANTE D'AGENTS ANTIMICROBIENS
Abstract
(EN) The invention relates to the use of co-expression of an antimicrobial agent and an enzyme, with a view to improving yield and/or overall production economy. Examples of antimicrobial agents are antimicrobial peptides such as lactoferricins and antimicrobial enzymes such as lysozyme and glucose oxidase, and examples of enzymes are endoglucanase, xylanase, phytase, protease, galactanase, mannanase, dextranase, alpha-galactosidase, pectate lyase, alpha-amylase and glucoamylase. A fusion product comprising the antimicrobial agent and the enzyme and a cleavable linker is novel, and can be used in animal feed and animal feed additives. The invention also describes the use of a protection domain wherein at least 50% of the amino acid residues comprised in the peptide protection domain are D (Asp) and/or E (Glu). The protection or quenching domain serves to temporarily and reversibly inactivate the antimicrobial peptide during the expression.
(FR) L'invention concerne l'utilisation de la co-expression d'un agent antimicrobien et d'une enzyme, dans le but d'améliorer le rendement et l'économie de production globale. Les agents antimicrobiens cités à titre d'exemple sont les peptides antimicrobiens, tels que les lactoferricines, et les enzymes antimicrobiennes, telles que le lysozyme et la glucose-oxydase, et les enzymes peuvent être, entre autres, l'endoglucanase, la xylanase, la phytase, la protéase, la galactanase, la mannanase, la dextranase, l'alpha-galactosidase, la pectate-lyase, l'alpha-amylase et la glucoamylase. Un produit de fusion comprenant l'agent antimicrobien et l'enzyme ainsi qu'un lieur est nouveau et peut être utilisé dans des aliments pour animaux et dans des additifs alimentaires pour animaux. L'invention porte également sur l'utilisation d'un domaine de protection, dans lequel au moins 50 % des résidus d'acide aminé compris dans le domaine de protection du peptide sont D (asp) et/ou E (Glu). Le domaine de protection ou d'extinction sert à inactiver temporairement et de manière réversible le peptide antimicrobien pendant l'expression.
Related patent documents
ZA200601544This application is not viewable in PATENTSCOPE because the national phase entry has not been published yet or the national entry is issued from a country that does not share data with WIPO or there is a formatting issue or an unavailability of the application.
US2007104764This application is not viewable in PATENTSCOPE because the national phase entry has not been published yet or the national entry is issued from a country that does not share data with WIPO or there is a formatting issue or an unavailability of the application.
Latest bibliographic data on file with the International Bureau