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1. WO2005021714 - LACCASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

Publication Number WO/2005/021714
Publication Date 10.03.2005
International Application No. PCT/US2004/025932
International Filing Date 11.08.2004
IPC
C07H 21/04 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04with deoxyribosyl as saccharide radical
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/53 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
C12P 19/34 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 1/21 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 5/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
CPC
A21D 8/042
AHUMAN NECESSITIES
21BAKING; EDIBLE DOUGHS
DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
8Methods for preparing or baking dough
02Methods for preparing dough; Treating dough prior to baking
04treating dough with microorganisms or enzymes
042with enzymes
A23C 9/1213
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
CDAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
9Milk preparations; Milk powder or milk powder preparations
12Fermented milk preparations; Treatment using microorganisms or enzymes
1203Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
1213Oxidation or reduction enzymes, e.g. peroxidase, catalase, dehydrogenase
A23C 9/152
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
CDAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
9Milk preparations; Milk powder or milk powder preparations
152containing additives
A23K 20/189
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
KFODDER
20Accessory food factors for animal feeding-stuffs
10Organic substances
189Enzymes
A23K 40/20
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
KFODDER
40Shaping or working-up of animal feeding-stuffs
20by moulding, e.g. making cakes or briquettes
A23K 40/25
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
KFODDER
40Shaping or working-up of animal feeding-stuffs
25by extrusion
Applicants
  • DIVERSA CORPORATION [US]/[US] (AllExceptUS)
  • HITCHMAN, Tim [US]/[US] (UsOnly)
  • ROBERTSON, Dan, E. [US]/[US] (UsOnly)
  • HIRAIWA, Masao [JP]/[US] (UsOnly)
  • PHILLIPS, Yoko [US]/[US] (UsOnly)
  • GRAY, Kevin, A. [US]/[US] (UsOnly)
Inventors
  • HITCHMAN, Tim
  • ROBERTSON, Dan, E.
  • HIRAIWA, Masao
  • PHILLIPS, Yoko
  • GRAY, Kevin, A.
Agents
  • EINHORN, Gregory, P.
Priority Data
60/494,47211.08.2003US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) LACCASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
(FR) LACCASES, ACIDES NUCLEIQUES CODANT POUR CES ENZYMES ET PROCEDES PERMETTANT DE LES PRODUIRE ET DE LES UTILISER
Abstract
(EN) The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1- hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
(FR) La présente invention concerne des laccases, des polynucléotides codant pour ces enzymes, ainsi que l'utilisation de tels polynucléotides et de polypeptides. Un aspect de cette invention concerne la production enzymatique de nootkatone par conversion du valencène au moyen de protéines présentant une activité de laccase, par exemple, une nouvelle laccase décrite dans cette invention. Un autre aspect de cette invention concerne des procédés permettant de dépolymériser la lignine, par exemple, lors d'un processus de fabrication de papier ou de pulpe, au moyen d'un polypeptide décrit dans cette invention. Un autre aspect de cette invention concerne des procédés permettant d'oxyder des produits qui peuvent être des médiateurs de réactions d'oxydation catalysées par laccases, par exemple, 2,2-azinobis-(3-éthylbenzthiazoline-6-sulfonate) (ABTS), 1- hydroxybenzotriazole (HBT), 2,2,6,6-tetraméthylpipéridin-1-yloxy (TEMPO), diméthoxyphénol, etc.
Latest bibliographic data on file with the International Bureau