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1. (WO2005012572) SCREENING METHOD OF REASSORTANT INFLUENZA VIRUSES USING PRIMER DEPENDENT MULTIPLEX RT-PCR
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/012572    International Application No.:    PCT/KR2004/001903
Publication Date: 10.02.2005 International Filing Date: 28.07.2004
Chapter 2 Demand Filed:    02.02.2005    
IPC:
C12Q 1/68 (2006.01), C12Q 1/70 (2006.01)
Applicants: CJ CORP. [KR/KR]; 500 Namdaemunno 5-ga, Jung-gu, Seoul 100-802 (KR) (For All Designated States Except US).
PROTHEON CO., LTD. [KR/KR]; 134 Sinchon-dong, Seodaemun-gu, Seoul 120-140 (KR) (For All Designated States Except US).
LEE, Jang Yun [KR/KR]; (KR) (For US Only).
KIM, Jae Seung [KR/KR]; (KR) (For US Only).
KIM, Hyun Ah [KR/KR]; (KR) (For US Only).
KIM, Yeon Hee [KR/KR]; (KR) (For US Only).
HA, Suk Hoon [KR/KR]; (KR) (For US Only).
LEE, Nam Joong [KR/KR]; (KR) (For US Only).
PARK, Wan Je [KR/KR]; (KR) (For US Only).
MOON, Moo Sang [KR/KR]; (KR) (For US Only).
SEONG, Baik Lin [KR/KR]; (KR) (For US Only).
LEE, Kwang Hee [KR/KR]; (KR) (For US Only)
Inventors: LEE, Jang Yun; (KR).
KIM, Jae Seung; (KR).
KIM, Hyun Ah; (KR).
KIM, Yeon Hee; (KR).
HA, Suk Hoon; (KR).
LEE, Nam Joong; (KR).
PARK, Wan Je; (KR).
MOON, Moo Sang; (KR).
SEONG, Baik Lin; (KR).
LEE, Kwang Hee; (KR)
Agent: SON, Min; 19th Floor, City Air Tower, 159-9 Samsung-dong, Kangnam-gu,, Seoul 135-973 (KR)
Priority Data:
10-2003-0053534 01.08.2003 KR
Title (EN) SCREENING METHOD OF REASSORTANT INFLUENZA VIRUSES USING PRIMER DEPENDENT MULTIPLEX RT-PCR
(FR) METHODE DE CRIBLAGE DE VIRUS REASSORTIS DE LA GRIPPE AU MOYEN D'UNE RT-PCR MULTIPLEXE DEPENDANTE DE L'AMORCE
Abstract: front page image
(EN)Disclosed is a screening method of genes of reassortant influenza viruses in a reassortant influenza virus preparation process by using a difference between primers in binding specificity to templates in multiplex RT-PCR. The screening method is characterized by simultaneously performing two or three PCRs in a single tube using primers that are designed to correspond to poorly conserved regions selected by a homology search between nucleotide sequences of genes of an attenuated influenza virus and those of a virulent virus, thereby effectively screening a 6:2 reassortant virus containing six internal genes from an attenuated influenza virus and two external genes from a virulent virus.
(FR)L'invention concerne une méthode de criblage de gènes de virus réassortis de la grippe dans un processus de préparation de virus de la grippe réassortis au moyen d'une différence entre les amorces de fixation spécifiques à des gabarits en RT-PCR multiplexe. Cette méthode de criblage consiste, de façon caractéristique, à exécuter simultanément deux ou trois PCR dans un tube unique au moyen d'amorces correspondant à des régions faiblement conservées sélectionnées par une recherche d'homologie entre des séquences de nucléotides de gènes de virus de la grippe atténués et celles de virus virulents, ce qui permet de cribler efficacement un virus réassorti 6 :2 contenant six gènes internes provenant d'un virus de la grippe atténué et deux gènes externes provenant d'un virus virulent.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: Korean (KO)