WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2005012482) ASSAY FOR IDENTIFICATION OF BIOACTIVE COMPOUNDS THAT INTERACT WITH HEAT SHOCK PROTEIN 90
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2005/012482    International Application No.:    PCT/US2004/021297
Publication Date: 10.02.2005 International Filing Date: 30.06.2004
Chapter 2 Demand Filed:    26.10.2005    
IPC:
G01N 21/21 (2006.01), G01N 33/52 (2006.01), G01N 33/53 (2006.01), G01N 33/566 (2006.01), G01N 33/567 (2006.01)
Applicants: SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH [US/US]; 1275 York Avenue, New York, NY 10021 (US) (For All Designated States Except US).
CHIOSIS, Gabriela [RO/US]; (US) (For US Only).
ROSEN, Neal [US/US]; (US) (For US Only).
HUEZO, Henri [US/US]; (US) (For US Only)
Inventors: CHIOSIS, Gabriela; (US).
ROSEN, Neal; (US).
HUEZO, Henri; (US)
Agent: LARSON, Marina, T.; MARINA LARSON & ASSOCIATES, LLC, P.o. Box 4928, Dillon, US 80435-4928 (US)
Priority Data:
60/483,806 30.06.2003 US
Title (EN) ASSAY FOR IDENTIFICATION OF BIOACTIVE COMPOUNDS THAT INTERACT WITH HEAT SHOCK PROTEIN 90
(FR) ESSAI D'IDENTIFICATION DE COMPOSES BIOACTIFS QUI INTERAGISSENT AVEC LA PROTEINE DU STRESS 90
Abstract: front page image
(EN)A method for evaluation of molecules to identify those that can act as therapeutic inhibitors of Hsp90 makes use of a fluorescence polarization (FP) assay and a cell based assay, either individually or in combination. The FP assay uses a fluorescently-labeled Hsp90 binding agent and measure the degree of fluorescence polarization relative to the standard. A decrease in the degree of polarization indicates that fluorescently-labeled Hsp90 binding agent has been wholly or partially displaced by a candidate molecule, and identifies the molecule as having activity as an inhibitor of Hsp90. The cell based assay tests for decrease is an Hsp90-dependent activity of normal or tumor cells.
(FR)Cette invention concerne un procédé d'évaluation de molécules susceptibles d'agir en tant qu'inhibiteurs thérapeutiques de Hsp90, reposant sur l'emploi d'une analyse à polarisation de fluorescence (FP) et d'une analyse cellulaire, seules ou combinées. L'analyse FP fait intervenir un agent de liaison Hsp90 à marquage fluorescent pour mesure le degré de polarisation de fluorescence par apport à une valeur type. Une baisse du degré de polarisation indique que l'agent de liaison Hsp90 à marquage fluorescent a été entièrement ou partiellement déplacé par une molécule candidate et désigne une molécule agissant comme inhibiteur permet de déterminer une baisse de l'activité dépendant de Hsp90 de cellules normales ou tumorales.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)