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Machine translation
1. (WO2004097428) SCREENING METHOD FOR IDENTIFYING HSP90 MODULATORS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/097428    International Application No.:    PCT/GB2004/001828
Publication Date: 11.11.2004 International Filing Date: 28.04.2004
IPC:
G01N 33/68 (2006.01)
Applicants: UNIVERSITY OF LIVERPOOL [GB/GB]; Senate House, Abercromby Square, Liverpool L69 3BX (GB) (For All Designated States Except US).
JENKINS, John [GB/GB]; (GB) (For US Only).
GREENHALF, William [GB/GB]; (GB) (For US Only).
O'CONNOR, David, Ian [GB/GB]; (GB) (For US Only)
Inventors: JENKINS, John; (GB).
GREENHALF, William; (GB).
O'CONNOR, David, Ian; (GB)
Agent: BANFORD, Clifford, Paul; Marks & Clerk, 43 Park Place, Leeds LS1 2RY (GB)
Priority Data:
0310017.9 01.05.2003 GB
Title (EN) SCREENING METHOD FOR IDENTIFYING HSP90 MODULATORS
(FR) PROCEDE DE CRIBLAGE PERMETTANT L'IDENTIFICATION DE MODULATEURS DE HSP90
Abstract: front page image
(EN)A screening method for identifying and/or analysing Hsp90 inhibitors and/or Hsp90 agonists comprises the steps of contacting a compound with at least two of yeast strains A - E wherein each yeast strain comprises expression vectors from which a pair of binding partners for a yeast two-hybrid assay are expressed. The binding partner pairs comprise: A: Hsp90-targeting protein; B: Hsp90-Hsp9O; C: Hsp90-p23; D: Hsp90-E3 ligase; E: Hsp90-Client. Inhibition and/or promotion of dimerisation between the binding partners is then measured.
(FR)La présente invention concerne un procédé de criblage permettant d'identifier et/ou d'analyser des inhibiteurs de Hsp90 et/ou des agonistes de Hsp90. Ce procédé comprend les étapes consistant à mettre en contact un composé avec au moins deux souches de levure A - E telles que chaque souche de levure comprend des vecteurs d'expression à partir desquels sont exprimés deux partenaires de liaison pour une bioanalyse de levure à deux hybrides. Les paires de partenaires de liaison comprennent: A: Hsp90-protéine de ciblage; B: Hsp90-Hsp90; C: Hsp90-p23; D: Hsp90-ligase E3; E: Hsp90-client. L'inhibition et/ou l'activation de la dimérisation entre les partenaires de liaison est ensuite mesurée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)