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1. (WO2004094986) SYSTEM AND METHOD FOR ELECTROCHEMICAL DETECTION OF BIOLOGICAL COMPOUNDS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2004/094986 International Application No.: PCT/US2004/011900
Publication Date: 04.11.2004 International Filing Date: 16.04.2004
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
HANDYLAB, INC. [US/US]; 5230 S. State Road Suite 100 Ann Arbor, MI 48108-9754, US (AllExceptUS)
ALTHAUS, John, S. [US/US]; US (UsOnly)
KYONGHOON, Lee [KR/US]; US (UsOnly)
NAMASIVAYAM, Vijay [IN/US]; US (UsOnly)
BRAHMASANDRA, Sundaresh, N. [IN/US]; US (UsOnly)
HANDIQUE, Kalyan [IN/US]; US (UsOnly)
Inventors:
ALTHAUS, John, S.; US
KYONGHOON, Lee; US
NAMASIVAYAM, Vijay; US
BRAHMASANDRA, Sundaresh, N.; US
HANDIQUE, Kalyan; US
Agent:
FISTER, Julius, C., III; Fish & Richardson P.C. 1425 K Street, NW Washington, DC 20005, US
Priority Data:
60/463,04716.04.2003US
Title (EN) SYSTEM AND METHOD FOR ELECTROCHEMICAL DETECTION OF BIOLOGICAL COMPOUNDS
(FR) SYSTEME ET PROCEDE DE DETECTION ELECTROCHIMIQUE DE COMPOSES BIOLOGIQUES
Abstract:
(EN) The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reverisbly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.
(FR) La présente invention concerne un procédé électrochimique de détection d'un polynucléotide cible. Une électrode comprenant une surface d'électrode est prévue. La surface d'électrode comprend au moins une molécule sonde immobilisée de manière réversible par rapport à la surface d'électrode. Un premier signal électrochimique indiquant une quantité de molécule sonde immobilisée par rapport à la surface d'électrode est obtenu. La surface d'électrode est mise en contact avec un liquide comprenant le polynucléotide cible. Lors de l'étape de mise en contact, au moins une partie de la molécule sonde immobilisée par rapport à la surface d'électrode se dissocie de celle-ci. Un second signal électrochimique indiquant une quantité de molécule sonde immobilisée par rapport à la surface d'électrode est obtenu. La présence du polynucléotide cible est déterminée au moins partiellement sur la base des premier et second signaux électrochimiques.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)