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1. (WO2004094668) OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS

Pub. No.:    WO/2004/094668    International Application No.:    PCT/US2004/011885
Publication Date: Fri Nov 05 00:59:59 CET 2004 International Filing Date: Sat Apr 17 01:59:59 CEST 2004
IPC: C12M 1/34
C12Q 1/68
G01N 33/543
Applicants: BECKMAN COULTER, INC.
KEYS, Daniel, A.
REDDY, Parameswara, Meda
BODNAR, Jackie, S.
FAROOQUI, Firdous
Inventors: KEYS, Daniel, A.
REDDY, Parameswara, Meda
BODNAR, Jackie, S.
FAROOQUI, Firdous
Title: OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS
Abstract:
The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.