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1. (WO2004094668) OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2004/094668 International Application No.: PCT/US2004/011885
Publication Date: 04.11.2004 International Filing Date: 16.04.2004
Chapter 2 Demand Filed: 04.04.2005
IPC:
C12M 1/34 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 33/543 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
Applicants: KEYS, Daniel, A.[US/US]; US (UsOnly)
REDDY, Parameswara, Meda[US/US]; US (UsOnly)
BODNAR, Jackie, S.[US/US]; US (UsOnly)
FAROOQUI, Firdous[US/US]; US (UsOnly)
BECKMAN COULTER, INC.[US/US]; 4300 North Harbor Boulevard P.O. Box 3100 Fullerton, CA 92834-3100, US (AllExceptUS)
Inventors: KEYS, Daniel, A.; US
REDDY, Parameswara, Meda; US
BODNAR, Jackie, S.; US
FAROOQUI, Firdous; US
Agent: KARISH, Marc ; SHELDON & MAK PC 225 South Lake Avenue, 9th Floor Pasadena, CA 91101, US
PERREY, Ralf; Müller-Boré & Partner Grafinger Strasse 2 81671 Munich, DE
Priority Data:
10/419,02018.04.2003US
Title (EN) OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS
(FR) PAIRES D'OLIGONUCLEOTIDES POUR IMMUNODETECTION MULTIPLEXEE OLIGONUCLEOTIDE PAIRS FOR MULTIPLEXED BINDING ASSAYS
Abstract:
(EN) The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.
(FR) L'invention concerne un système de dosage basé sur les oligonucléotides et un nécessaire utile pour trier, détecter et identifier des substances à analyser. Le système met en oeuvre des paires d'oligonucléotides complémentaires dans lesquelles un oligonucléotide de chaque paire est immobilisé sur un substrat solide et un agent de liaison à la substance à analyser est fixé à l'autre oligonucléotide. Les différentes paires d'oligonucléotides s'hybrident sensiblement à la même vitesse, présentent le même Tm, présentent des séquences nucléotidiques mises au point pour minimiser l'hybridation croisée entre paires différentes, et s'hybrident entre elles relativement rapidement à températures ambiantes, sans hybridation croisée décelable.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)