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1. (WO2004093645) TN5 TRANSPOSASE MUTANTS AND THE USE THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/093645    International Application No.:    PCT/US2004/011879
Publication Date: 04.11.2004 International Filing Date: 16.04.2004
IPC:
C12N 15/87 (2006.01)
Applicants: WISCONSIN ALUMNI RESEARCH FOUNDATION [US/US]; 614 Walnut Street, Madison, WI 53707-7365 (US) (For All Designated States Except US).
REZNIKOFF, William, S. [US/US]; (US) (For US Only).
STEINGER-WHITE, Mindy, M. [US/US]; (US) (For US Only).
METZLER, Jeremy, D. [US/US]; (US) (For US Only)
Inventors: REZNIKOFF, William, S.; (US).
STEINGER-WHITE, Mindy, M.; (US).
METZLER, Jeremy, D.; (US)
Agent: REN, Zhibin; Quarles & Brady LLP, 411 East Wisconsin Avenue, Milwaukee, WI 53202 (US)
Priority Data:
60/463,470 17.04.2003 US
Title (EN) TN5 TRANSPOSASE MUTANTS AND THE USE THEREOF
(FR) MUTANTS DE TRANSPOSASE TN5 ET LEUR UTILISATION
Abstract: front page image
(EN)Tn5 transposase (Tnp) mutants that have higher transposase activities than the wild-type Tnp are disclosed. The Tn5 mutants differ from the wild-type Tnp at amino acid positions 54, 242, and 372 and have greater avidity than the wild-type Tnp for at least one of a wild-type Tn5 outside end sequence as defined by SEQ ID NO:3 and a modified Tn5 outside end sequence as defined by SEQ ID NO:5. Also disclosed are various systems and methods of using the Tnp mutants for in vitro or in vivo transposition.
(FR)L'invention concerne des mutants de transposase (Tnp) Tn5 présentant une activité de transposase plus élevée que celle de la Tnp du type sauvage. Les mutants de TnpTn5 diffèrent de la Tnp du type sauvage aux positions d'acide aminé 54, 242 et 372 et présentent, par rapport à la Tnp du type sauvage, une avidité supérieure vis-à-vis d'une séquence d'extrémité extérieure de Tn5 du type sauvage telle que définie par SEQ ID NO:3 et/ou une séquence d'extrémité extérieure de Tn5 modifiée telle que définie par SEQ ID NO:5. L'invention concerne également différents systèmes et procédés pour l'utilisation des mutants de Tnp à des fins de transposition <i>in vitro </i>ou<i> in vivo.</i>
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)