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1. (WO2004088283) APPARATUS AND METHODS FOR PROVIDING SEX-SORTED ANIMAL SPERM
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2004/088283 International Application No.: PCT/US2004/009646
Publication Date: 14.10.2004 International Filing Date: 29.03.2004
IPC:
A01N 1/02 (2006.01) ,C12M 1/34 (2006.01) ,C12N 5/071 (2010.01) ,C12Q 1/04 (2006.01) ,G01N 1/30 (2006.01) ,G01N 15/00 (2006.01) ,G01N 15/14 (2006.01) ,G01N 33/48 (2006.01) ,G01N 33/487 (2006.01) ,G01N 33/50 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
02
Preservation of living parts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
30
Staining; Impregnating
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15
Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10
Investigating individual particles
14
Electro-optical investigation
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
487
of liquid biological material
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Applicants:
MONSANTO TECHNOLOGY LLC [US/US]; 800 North Lindbergh Boulevard St. Louis, Missouri 63102, US (AllExceptUS)
DURACK, Gary [US/US]; US (UsOnly)
HATCHER, Jeremy, T. [US/US]; US (UsOnly)
WESTFALL, Lon, A. [US/US]; US (UsOnly)
HELBLING, David, R. [US/US]; US (UsOnly)
WALLACE, Jeffrey, D. [US/US]; US (UsOnly)
VANDRE, Gary, P. [US/US]; US (UsOnly)
DIDION, Bradley [US/US]; US (UsOnly)
NAYAK, Niraj, V. [US/US]; US (UsOnly)
ANZAR, Muhammad [CA/US]; US (UsOnly)
LUDWIG, Cindy, L. [US/US]; US (UsOnly)
GRAHAM, Jeffrey, A. [US/US]; US (UsOnly)
CROWLEY, Kathleen, S. [US/US]; US (UsOnly)
Inventors:
DURACK, Gary; US
HATCHER, Jeremy, T.; US
WESTFALL, Lon, A.; US
HELBLING, David, R.; US
WALLACE, Jeffrey, D.; US
VANDRE, Gary, P.; US
DIDION, Bradley; US
NAYAK, Niraj, V.; US
ANZAR, Muhammad; US
LUDWIG, Cindy, L.; US
GRAHAM, Jeffrey, A.; US
CROWLEY, Kathleen, S.; US
Agent:
GODAR, Michael, E. ; Senniger, Powers, Leavitt & Roedel #1 Metropolitan Square, 16th Floor St. Louis, Missouri 63102, US
Priority Data:
60/458,60728.03.2003US
60/458,73128.03.2003US
Title (EN) APPARATUS AND METHODS FOR PROVIDING SEX-SORTED ANIMAL SPERM
(FR) DISPOSITIFS, PROCEDES ET PROCESSUS PERMETTANT DE TRIER DES PARTICULES ET D'OBTENIR DU SPERME ANIMAL PRODUISANT UNE PROGENITURE DE SEXE DONNE
Abstract:
(EN) Apparatus and methods for analyzing particles, including apparatus and methods for a sperm sorting process including: collecting sperm from an animal (30); selecting staining conditions (47A); staining the sperm with DNA selective fluorescent dye (48); sorting the sperm cells according to sex chromosome content (55); and cryopreserving a population of sorted sperm (61) until used for artificial insemination. One embodiment includes apparatus (1001) and methods for using a plurality of flow cytometry units (9) sharing an integrated platform to sort sperm cells. In one embodiment, flow cytometric sorting includes use of the following apparatus and methods: an orienting nozzle having a baffle (101); an epi-illumination optics system (109); slit scanning of localized DNA regions within cell nuclei (225); digital signal processing, including synchronous sampling of analog output signals (701), pulse waveform (497) feature extraction of an approximation of a first order derivative of a pulse waveform (497) at a point of the pulse, any of various sort strategies; and an automated sort calibration system (4201). In one embodiment, digital signal processing includes sampling analog output signals (701) at times relative to emission of pulses from an illumination laser. Other embodiments are substantially different from the foregoing, including embodiments directed to individual steps or systems that can be used for any of various applications involving particle analysis.
(FR) L'invention concerne des dispositifs et des procédés permettant d'analyser des particules, y compris des dispositifs et des procédés de tri de sperme consistant : à collecter du sperme d'un animal (30) ; à sélectionner des conditions de coloration (47a) ; à colorer le sperme avec un colorant fluorescent ADN-sélectif (48) ; à trier les cellules spermatiques en fonction de la teneur en chromosomes sexuels (55) ; et à cryoconserver une population de sperme trié (61) jusqu'à son utilisation pour une insémination artificielle. Un mode de réalisation comprend un dispositif (1001) et des procédés permettant d'utiliser une pluralité d'unités de cytométrie de flux (9) partageant une plate-forme intégrée pour trier les cellules spermatiques. Dans un autre mode de réalisation, le tri cytométrique de flux consiste à utiliser les dispositifs et les procédés suivants : un embout orienteur comprenant une chicane (101) ; un système optique à éclairage vertical (109) ; à balayer par une fente les régions d'ADN localisées dans le noyau cellulaire (225) ; à réaliser un traitement numérique du signal, comprenant un échantillonnage synchrone de signaux de sortie analogiques (701), une extraction de caractéristiques de forme d'impulsion (497) d'une approximation d'une dérivée de premier ordre d'une forme d'impulsion (497) à un point de l'impulsion, ainsi que diverses stratégies de tri ; ainsi qu'un système d'étalonnage de tri automatisé (4201). Dans un autre mode de réalisation, le traitement numérique du signal consiste à échantillonner les signaux de sortie analogiques (701) à des instants données par rapport à l'émission d'impulsions par un laser éclairant. L'invention concerne également d'autres modes de réalisation sensiblement différents de ceux déjà cités, se rapportant à des étapes ou à des systèmes individuels pouvant être utilisés pour diverses applications nécessitant une analyse particulaire.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
NZ542547MXPA/a/2005/010492EP2194379EP1608963EP2298231EP2308416
EP2308417EP2308418EP2308419EP2309245EP2309246EP2305171
EP2305172EP2305173EP2305832EP2306173EP2306174EP2308420
EP2309244EP2332492ZA2005/07151JP2006524054EP2357464JP4614947
CN1795382CA2518882EP2959774DK1608963AU2004225436IN3937/DELNP/2005
BR122017017790BRPI0408857