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1. (WO2004033396) METHOD FOR ENRICHING ADHERENT MONOCYTE POPULATIONS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/033396    International Application No.:    PCT/US2003/031759
Publication Date: 22.04.2004 International Filing Date: 07.10.2003
IPC:
C12N 5/0786 (2010.01), A01N 1/02 (2006.01)
Applicants: AMERICAN NATIONAL RED CROSS [US/US]; 1811 Gatehouse Road, Falls Church, VA 22042 (US) (For All Designated States Except US).
WAGNER, Stephen, J. [US/US]; (US) (For US Only).
MYRUP, Andrew [US/US]; (US) (For US Only).
CELLUZZI, Christina, M. [US/US]; (US) (For US Only)
Inventors: WAGNER, Stephen, J.; (US).
MYRUP, Andrew; (US).
CELLUZZI, Christina, M.; (US)
Agent: O'SHAUGHNESSY, Brian, P.; Burns, Doane, Swecker & Mathis, L.L.P., P.O. BOX 1404, Alexandria, VA 22313-1404 (US)
Priority Data:
60/416,527 08.10.2002 US
Title (EN) METHOD FOR ENRICHING ADHERENT MONOCYTE POPULATIONS
(FR) PROCEDE D'ENRICHISSEMENT DE POPULATIONS DE MONOCYTES ADHERENTS
Abstract: front page image
(EN)The present invention provides methods and apparatus for the rapid and efficient collection and purification of activated monocytes. The methods and apparatus of the present invention provide means for effecting the collection and purification in an aseptic environment. The method involves filtering a blood component mixture through a monocyte-adhering filter; washing the blood component mixture; backflushing the filter with a physiological solution; and backflushing the filter with Dextran-40/serum albumin.
(FR)L'invention concerne des procédés et un appareil de collecte et de purification rapides et efficaces de monocytes activés. Les procédés et l'appareil de l'invention mettent en oeuvre des moyens de réalisation de la collecte et de la purification dans un environnement aseptique. Le procédé consiste à filtrer un mélange de composants sanguins via un filtre d'adhésion de monocytes, à laver le mélange des composants sanguins, à rincer le filtre à contre-courant avec une solution physiologique, et à rincer le filtre à contre-courant avec de la sérumalbumine/dextran-40.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)