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1. (WO2004013604) NOVEL PROCEDURE FOR GROWTH, IMAGING, AND ENUMERATION OF MICROBIAL COLONIES FOR SEROLOGICAL OR SCREENING ASSAYS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2004/013604 International Application No.: PCT/US2003/023928
Publication Date: 12.02.2004 International Filing Date: 30.07.2003
Chapter 2 Demand Filed: 11.02.2004
IPC:
C12M 1/12 (2006.01) ,C12M 1/18 (2006.01) ,C12N 1/20 (2006.01) ,C12N 11/02 (2006.01) ,C12Q 1/02 (2006.01) ,C12Q 1/04 (2006.01) ,C12Q 1/18 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
12
with sterilisation, filtration, or dialysis means
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
16
containing, or adapted to contain, solid media
18
Multiple fields or compartments
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11
Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02
Enzymes or microbial cells being immobilised on or in an organic carrier
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
18
Testing for antimicrobial activity of a material
Applicants:
MERCK & CO., INC. [US/US]; 126 East Lincoln Avenue Rahway, NJ 07065-0907, US (AllExceptUS)
CAULFIELD, Michael, J. [US/US]; US (UsOnly)
LIU, Xu [CN/US]; US (UsOnly)
WANG, Su [CN/US]; US (UsOnly)
Inventors:
CAULFIELD, Michael, J.; US
LIU, Xu; US
WANG, Su; US
Common
Representative:
MERCK & CO., INC.; 126 East Lincoln Avenue Rahway, NJ 07065-0907, US
Priority Data:
60/400,33201.08.2002US
Title (EN) NOVEL PROCEDURE FOR GROWTH, IMAGING, AND ENUMERATION OF MICROBIAL COLONIES FOR SEROLOGICAL OR SCREENING ASSAYS
(FR) PROCEDE ASSURANT LA CROISSANCE, L'IMAGERIE ET L'ENUMERATION DE COLONIES MICROBIENNES POUR LES ESSAIS DE SEROLOGIE OU D'ANALYSE
Abstract:
(EN) A novel method is provided, for use within serological or screening assays, wherein microbial colonies are grown on filter membranes in multi-well plates. This process enables the colonies to be stained, imaged, and counted automatically using such automated systems as, e.g., computer and video-based imaging systems.
(FR) L'invention concerne un procédé utilisable pour les essais de sérologie ou d'analyse, qui permet d'assurer la croissance de colonies microbiennes sur des membranes filtrantes de plaques à puits multiples, c'est-à-dire d'assurer la coloration, l'imagerie et l'énumération automatiques des colonies par le biais de systèmes automatiques, du type systèmes d'imagerie informatisés et vidéo.
front page image
Designated States: CA, JP, US
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1539993US20060063225CA2494033