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1. (WO2004010101) DETECTION OF CHEMICAL LIGATION USING FLUORESCENCE QUENCHING LEAVING GROUPS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/010101    International Application No.:    PCT/US2003/022320
Publication Date: 29.01.2004 International Filing Date: 17.07.2003
Chapter 2 Demand Filed:    13.02.2004    
IPC:
G01N 33/58 (2006.01)
Applicants: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY [US/US]; 1705 El Camino Real, Palo Alto, CA 94306-1106 (US)
Inventors: KOOL, Eric T.,; (US)
Agent: BUNTEL, Christopher J.,; HOWREY SIMON ARNOLD & WHITE LLP, 750 Bering Drive, Houston, TX 77057-2198 (US)
Priority Data:
60/396,774 18.07.2002 US
Title (EN) DETECTION OF CHEMICAL LIGATION USING FLUORESCENCE QUENCHING LEAVING GROUPS
(FR) DETECTION DE LIGATURES CHIMIQUES A L'AIDE DE GROUPES PARTANTS D'EXTINCTION DE FLUORESCENCE
Abstract: front page image
(EN)Novel compounds having a fluorescence quencher as a leaving group are disclosed. Nucleic acids and other molecules containing a fluorophore and a fluorescence quencher are disclosed as an embodiment of this invention. The use of the oligonucleotides in enzyme-free oligonucleotide ligation reactions results in an increase in fluorescence when the oligonucleotide also contains a nearby fluorophore. The ligation reactions can be performed in solution, on surfaces, or in cells.
(FR)Cette invention se rapporte à de nouveaux composés comportant un agent d'extinction de fluorescence comme groupe partant. Des acides nucléiques et d'autres molécules contenant un fluorophore et un agent d'extinction de fluorescence sont décrits comme mode de réalisation de cette invention. L'utilisation des oligonucléotides dans des réactions de ligature d'oligonucléotides sans enzyme entraîne une augmentation de la fluorescence, lorsque l'oligonucléotide contient également un fluorophore voisin. Les réactions de ligature peuvent être effectuées en solution, sur des surfaces ou dans des cellules.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)