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Machine translation
1. (WO2004007081) LOW DEAD VOLUME EXTRACTION COLUMN DEVICES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/007081    International Application No.:    PCT/US2003/021998
Publication Date: 22.01.2004 International Filing Date: 14.07.2003
IPC:
B01L 3/02 (2006.01), G01N 30/00 (2006.01), G01N 30/60 (2006.01), G01N 35/10 (2006.01)
Applicants: PHYNEXUS, INC. [--/US]; 3670 Charter Park Drive, Suite A San Jose, CA 95136 (US) (For All Designated States Except US).
GJERDE, Douglas, T. [US/US]; (US) (For US Only).
HANNA, Christopher, P. [US/US]; (US) (For US Only)
Inventors: GJERDE, Douglas, T.; (US).
HANNA, Christopher, P.; (US)
Agent: HOLMAN, Christopher, M.; PhyNexus, Inc., IP Dept., 3670 Charter Park Drive, Suite A, San Jose, CA 95136 (US)
Priority Data:
60/396,595 15.07.2002 US
60/465,606 25.04.2003 US
Title (EN) LOW DEAD VOLUME EXTRACTION COLUMN DEVICES
(FR) DISPOSITIFS DE COLONNES D'EXTRACTION A FAIBLE VOLUME MORT
Abstract: front page image
(EN)The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.
(FR)L'invention concerne des colonnes d'extraction destinées à la purification d'un analyte (par exemple, une macromolécule biologique, telle qu'un peptide, une protéine ou un acide nucléique) contenu dans une solution de dosage, ainsi que des procédés de fabrication de ces colonnes. L'invention est caractérisée par l'utilisation de colonnes de faible volume mort, obtenues en partie par l'emploi de disques frittés à faible volume poreux (tels que les tamis membranaires) destinés à contenir un lit de milieu d'extraction dans la colonne. Le faible volume mort facilite l'élution de l'analyte capturé en un très faible volume de solution de désorption, ce qui permet de préparer des échantillons à faible volume présentant des concentrations élevées d'analyte.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)