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1. (WO2004005883) SECRETED AND CYTOPLASMIC TUMOR ENDOTHELIAL MARKERS

Pub. No.:    WO/2004/005883    International Application No.:    PCT/US2003/016250
Publication Date: Jan 15, 2004 International Filing Date: Jul 2, 2003
IPC: A61K 39/395
A61K 38/00
C12Q 1/68
G01N 33/53
Applicants: THE JOHNS HOPKINS UNIVERSITY
ST. CROIX, Brad
KINZLER, Kenneth, W.
VOGELSTEIN, Bert
Inventors: ST. CROIX, Brad
KINZLER, Kenneth, W.
VOGELSTEIN, Bert
Title: SECRETED AND CYTOPLASMIC TUMOR ENDOTHELIAL MARKERS
Abstract:
To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed many differentially expressed genes, including a large nujber of genes that were specifically elevated in tumor-associated endothelium. Experiments with representative genes from this group demonstrated that most were similarly expressed in the endothelium of primary lung, breast, brain, and pancreatic cancers as well as in metastatic lesions fo the liver. Theses results demonstrate that neoplastic and normal endothelium in humans are distinct at the molecular level, and have significant implications for the development of anti-angiogenic.