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1. WO2004003215 - METHOD OF ISOLATING BIOLOGICALLY ACTIVE FRACTION CONTAINING CLINICALLY ACCEPTABLE NATIVE SLIPOPOLYSACCHARIDES OBTAINED FROM BACTERIA PRODUCING ENDOTOXIC LIPOPOLYSACCHARIDES

Publication Number WO/2004/003215
Publication Date 08.01.2004
International Application No. PCT/RU2003/000250
International Filing Date 03.06.2003
Chapter 2 Demand Filed 26.01.2004
IPC
A61K 31/739 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
739Lipopolysaccharides
A61K 35/74 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
A61K 39/02 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
02Bacterial antigens
C08B 37/00 2006.01
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
BPOLYSACCHARIDES; DERIVATIVES THEREOF
37Preparation of polysaccharides not provided for in groups C08B1/-C08B35/110; Derivatives thereof
C12P 19/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
CPC
A61K 2039/6068
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
60characteristics by the carrier linked to the antigen
6031Proteins
6068Other bacterial proteins, e.g. OMP
A61K 31/739
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
739Lipopolysaccharides
A61K 35/74
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
74Bacteria
A61K 39/0283
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
02Bacterial antigens
025Enterobacteriales, e.g. Enterobacter
0283Shigella
A61P 37/04
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
37Drugs for immunological or allergic disorders
02Immunomodulators
04Immunostimulants
C08B 37/00
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
BPOLYSACCHARIDES; DERIVATIVES THEREOF
37Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Applicants
  • APARIN, Petr Gennadievich [RU]/[RU]
  • LVOV, Vyacheslav Leonidovich [RU]/[RU]
  • ELKINA, Stanislava Ivanovna [RU]/[RU] (UsOnly)
  • GOLOVINA, Marina Eduardovna [RU]/[RU] (UsOnly)
  • SHMIGOL, Vladimir Igorevich [RU]/[RU] (UsOnly)
Inventors
  • APARIN, Petr Gennadievich
  • LVOV, Vyacheslav Leonidovich
  • ELKINA, Stanislava Ivanovna
  • GOLOVINA, Marina Eduardovna
  • SHMIGOL, Vladimir Igorevich
Agents
  • EGOROVA, Galina Borisovna
Priority Data
200211705526.06.2002RU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF ISOLATING BIOLOGICALLY ACTIVE FRACTION CONTAINING CLINICALLY ACCEPTABLE NATIVE SLIPOPOLYSACCHARIDES OBTAINED FROM BACTERIA PRODUCING ENDOTOXIC LIPOPOLYSACCHARIDES
(FR) PROCEDE D'ISOLATION D'UNE FRACTION BIOLOGIQUEMENT ACTIVE CONTENANT DES S-LIPOPOLYSACCHARIDES NATURELS CLINIQUEMENT ACCEPTABLES, OBTENUS A PARTIR DE BACTERIES PRODUISANT DES LIPOPOLYSACCHARIDES ENDOTOXIQUES
Abstract
(EN)
A biologically active fraction (BAF) is presented containing mainly S-lipopolysaccharide (LPS) from gram-negative bacteria producing endotoxic LPSs. These fractions are characterized in that in the lipid A of S-LPS the mole ratio of D-glucosamine and &bgr; -hydroxy acids selected from the group comprising &bgr; -hydroxydecanoic, &bgr; -hydroxydodecanoic, (&bgr;-hydroxyhexadecanoic, (&bgr; hydroxyhexadecanoic, is approximately 2:1-4, and the mole ratio of D-glucosamine and higher fatty acids, connected both by amide and ester links, in the lipid A of S-LPS is approximately 2:3-7. A method of isolating BAF, containing mainly S-LPS from gram-negative bacteria producing endotoxic S-LPSs, is also presented. The obtained BAFs have pyrogenicity at the level of commercial polysaccharide vaccines and low endotoxicity, have high immunogenicity, which makes it possible to use them as vaccines for mammals, including humans. They are an inducer of cytokines and also may be considered to be a prophylactic tolerogenic anti-shock preparation.
(FR)
L'invention concerne une fraction biologiquement active (BAF) contenant principalement des S-lipopolysaccharides (LPS) provenant de bactéries Gram négatif produisant des LPS endotoxiques. Ces fractions sont caractérisées en ce que dans le lipide A de S-LPS, le rapport molaire de D-glucosamine et des acides $g(b)-hydroxy sélectionnés dans le groupe comprenant les acides $g(b)-hydroxydécanoïque, $g(b) -hydroxydodécanoïque, $g(b)-hydroxyhexadécanoïque, $g(b)-hydroxyhexadécanoïque, est approximativement de 2 : 1-4, et le rapport molaire de D-glucosamine et des acides gras supérieurs, liés à la fois par les liaisons amide et ester, dans le lipide A de S-LPS est approximativement de 2 : 3-7.:3-7. L'invention concerne également un procédé d'isolation de BAF contenant principalement S-LPS, à partir de bactéries Gram négatif produisant des S-LPS endotoxiques. Les BAF obtenus ont une pyrogénicité du niveau des vaccins polysaccharide de type commercial ; ils présentent par ailleurs une faible endotoxicité et une immunogénicité élevée, ce qui rend possible leur utilisation comme vaccins pour des mammifères, y compris des êtres humains. Ils constituent un inducteur des cytokines et peuvent également être envisagés pour une préparation anti-choc prophylactique tolérogène.
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