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1. (WO2004003200) ISOLATION OF NUCLEIC ACIDS USING A POLYCATIONIC POLYMER AS PRECIPITATION AGENT
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/003200    International Application No.:    PCT/SE2003/001127
Publication Date: 08.01.2004 International Filing Date: 26.06.2003
Chapter 2 Demand Filed:    12.01.2004    
IPC:
C07H 1/06 (2006.01), C07H 1/08 (2006.01), C12N 15/10 (2006.01)
Applicants: AMERSHAM BIOSCIENCES AB [SE/SE]; Björkgatan 30, S-751 84 Uppsala (SE) (For All Designated States Except US).
GALAEV, Igor, Yu. [RU/SE]; (SE) (For US Only).
GUSTAVSSON, Per-Erik [SE/SE]; (SE) (For US Only).
IZUMRUDOV, Vladimir, A. [RU/RU]; (RU) (For US Only).
LARSSON, Per-Olof [SE/SE]; (SE) (For US Only).
WAHLUND, Per-Olof [SE/SE]; (SE) (For US Only)
Inventors: GALAEV, Igor, Yu.; (SE).
GUSTAVSSON, Per-Erik; (SE).
IZUMRUDOV, Vladimir, A.; (RU).
LARSSON, Per-Olof; (SE).
WAHLUND, Per-Olof; (SE)
Agent: KILANDER, Annika; Amersham Biosciences AB, Patent Department, Björkgatan 30, S-751 84 Uppsala, Björkgatan 30 (SE)
Priority Data:
0202074-1 28.06.2002 SE
0301034-5 08.04.2003 SE
Title (EN) ISOLATION OF NUCLEIC ACIDS USING A POLYCATIONIC POLYMER AS PRECIPITATION AGENT
(FR) ISOLATION D'ACIDES NUCLEIQUES A L'AIDE D'UN POLYMERE POLYCATIONIQUE UTILISE EN TANT QU'AGENT DE PRECIPITATION
Abstract: front page image
(EN)The present invention relates to a method of isolating a nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+] / [-] between polycationic precipitating agent and nucleic acid is ≥ 0.5, preferably ≥ 0.9 and most preferably ≥1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nucleic acid/polycation complex.
(FR)La présente invention concerne une méthode d'isolation d'un acide nucléique d'une solution biologique, ladite méthode consistant à précipiter de manière sélective l'acide nucléique désiré par addition d'un agent de précipitation polycationique dans la solution, en lui permettant de former un complexe avec ledit acide nucléique, l'agent de précipitation étant un polymère linéaire hautement chargé contenant des groupes amino quaternaires. La quantité d'agent de précipitation polycationique ajoutée est, de préférence, telle que le rapport de charge [+] / [-] entre l'agent de précipitation polycationique et l'acide nucléique est = 0,5, de préférence = 0,9 et, plus préférablement, = 1 au cours de la précipitation, et en présence d'une concentration de sels garantissant la précipitation quantitative spécifique du complexe acide nucléique/polycation.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)