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1. (WO2004003135) SELECTIVE CLONING OF HOMODUPLEX NUCLEIC ACIDS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2004/003135    International Application No.:    PCT/US2003/019575
Publication Date: 08.01.2004 International Filing Date: 20.06.2003
Chapter 2 Demand Filed:    26.01.2004    
IPC:
C12N 9/22 (2006.01), C12N 15/10 (2006.01)
Applicants: STRATAGENE [US/US]; 11011 North Torrey Pines Road, La Jolla, CA 92037 (US) (For All Designated States Except US).
GREENER, Alan, L. [US/US]; (US) (For US Only).
HEXDALL, Lisa, Joy [US/US]; (US) (For US Only).
CARSTENS, Carsten-Peter [US/US]; (US) (For US Only).
SORGE, Joseph, A. [US/US]; (US) (For US Only)
Inventors: GREENER, Alan, L.; (US).
HEXDALL, Lisa, Joy; (US).
CARSTENS, Carsten-Peter; (US).
SORGE, Joseph, A.; (US)
Agent: WILLIAMS, Kathleen, M.; Palmer & Dodge LLP, 111 Huntington Avenue, Boston, MA 02199-7613 (US)
Priority Data:
10/180,174 26.06.2002 US
10/246,838 19.09.2002 US
Title (EN) SELECTIVE CLONING OF HOMODUPLEX NUCLEIC ACIDS
(FR) CLONAGE SELECTIF D'ACIDES NUCLEIQUES HOMODUPLEX
Abstract: front page image
(EN)The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
(FR)La présente invention porte sur un procédé de clonage sélectif de molécules d'acide nucléique homoduplex, notamment en utilisant une souche de cellules hôtes qui contient une enzyme exprimée sous condition et/ou une enzyme active reconnaissant des mésappariements sous condition, par ex., un variant sensible à la température du gène codant l'endonucléase VII à partir du phage T4. A l'aide de cette souche hôte, l'invention met en avant un procédé de clonage qui sélectionne des produits PCR qui sont dépourvus de mutations générées par la PCR.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)