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1. (WO2003106623) EARLY NONINVASIVE PRENATAL TEST FOR ANEUPLOIDIES AND HERITABLE CONDITIONS
Latest bibliographic data on file with the International Bureau   
Pub. No.: WO/2003/106623 International Application No.: PCT/US2003/018271
Publication Date: 24.12.2003 International Filing Date: 11.06.2003
IPC:
C12Q 1/68 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/554 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
554
the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
Applicants:
NEW YORK UNIVERSITY [US/US]; 70 Washington Square South New York, NY 10012, US
Inventors:
THOMAS, John, O.; US
Agent:
BROWDY, Roger, L. ; Browdy and Neimark, P.L.L.C. 624 Ninth Street N.W. Suite 300 Washington, DC 20001-5303, US
Priority Data:
60/387,91413.06.2002US
Title (EN) EARLY NONINVASIVE PRENATAL TEST FOR ANEUPLOIDIES AND HERITABLE CONDITIONS
(FR) TESTE PRECOCE PRENATAL NON EFFRACTIF POUR DETECTER DES ANEUPLOIDIES ET DES ETATS PATHOLOGIQUES HEREDITAIRES
Abstract:
(EN) The present invention provides methods for isolating fetal cells from samples of maternal blood, and for detecting aneuploidies and heritable disorders. Fetal cells are enriched from maternal blood samples using layered immunosorption to specifically bind erythroid cell precursors. In the layered immunosorption method, a substrate such as a microscope slide is coated with a thin layer of erythrocyte membranes. Prior to assay the membranes are activated by binding an antibody against an erythroid cell surface protein. The sample is then added to the substrate and incubated. Nonadsorbed cells are removed by washing, and the bound erythroid cells are permanently attached by fixation and drying. Molecular beacons or other molecular probes are used for differentially detecting fetal and maternal cells. Permeabilizing detergents are used for purifying and detecting fetal cells.
(FR) L'invention concerne des procédés pour isoler des cellules foetales à partir d'échantillons de sang maternel et pour détecter des aneuploïdies et des troubles héréditaires. Des cellules foetales sont enrichies à partir d'échantillons de sang maternel par immuniadsorption de manière à lier de manière spécifique des précurseurs de cellules érythroides. Dans le procédé d'immunoadsorption en couches, un substrat tel qu'une lame de microscope est recouverte par une couche fine de membranes érythrocytes. Avant les essais biologiques, lesdites membranes sont activées par liaison d'un anticorps avec une protéine de la surface des cellules érythrocytes. Ledit échantillon est ensuite ajouté au substrat puis incubé. Les cellules non adsorbées sont éliminées lors de l'étape de lavage, et les cellules érythrocytes liées sont fixées de manière permanente par fixation et séchage. Des balises moléculaires et d'autres sondes moléculaires sont utilisées pour détecter de manière différentielle les cellules foetales et maternelles. Des détergents de perméabilisation sont utilisés pour purifier et détecter les cellules foetales.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)