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1. (WO2003076662) IDENTIFICATION OF MICROBIAL POLYNUCLEOTIDES EXPRESSED DURING INFECTION OF A HOST

Pub. No.:    WO/2003/076662    International Application No.:    PCT/US2003/006768
Publication Date: Sep 18, 2003 International Filing Date: Mar 5, 2003
IPC: C07H 21/04
C07K 16/00
C12Q 1/68
C12Q 1/70
G01N 33/53
G01N 33/543
Applicants: IVIGENE CORPORATION
HILLMAN, Jeffrey, D.
Inventors: HILLMAN, Jeffrey, D.
Title: IDENTIFICATION OF MICROBIAL POLYNUCLEOTIDES EXPRESSED DURING INFECTION OF A HOST
Abstract:
The invention provides methods useful for identifying polynucleotides expressed in vivo by a microbe during infection of a host. Antibodies against antigens that are expressed by the microbe in vivo and in vitro are absorbed with cells or cellular extracts of the microbe that have been grown in vitro. Unadsorbed antibodies are isolated and are probed against a phage display library of the microbe's DNA or RNA. A polynucleotide of the microbe that is expressed in vivo is isolated and identified.