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1. (WO2003062827) FLUORESCENT DETECTION OF PROTEINS IN POLYACRYLAMIDE GELS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2003/062827    International Application No.:    PCT/CA2003/000095
Publication Date: 31.07.2003 International Filing Date: 27.01.2003
IPC:
G01N 33/68 (2006.01)
Applicants: UNIVERSITY TECHNOLOGIES INTERNATIONAL INC. [CA/CA]; 3553 - 31 Street, N.W., Suite 130, Calgary, Alberta T2L 2K7 (CA)
Inventors: EDWARDS, Robert, A.; (CA).
TURNER, Raymond, J.; (CA).
LADNER, Carol; (CA).
STARKEY, Jean; (US).
KAZMIN, Dmitri; (US).
LARSON, Eric; (US)
Agent: BENNETT JONES LLP; 1000 ATCO Centre, 10035 - 105 Street, Edmonton, Alberta T5J 3T2 (CA)
Priority Data:
60/319,099 25.01.2002 US
60/352,225 29.01.2002 US
60/319,810 24.12.2002 US
Title (EN) FLUORESCENT DETECTION OF PROTEINS IN POLYACRYLAMIDE GELS
(FR) DETECTION PAR FLUORESCENCE DE PROTEINES DANS DES GELS DE POLYACRYLAMIDE
Abstract: front page image
(EN)The UV light-induced reaction between the indole moiety of tryptophan (Trp) and haloalkanes permits rapid visualization of protein resolved by polyacrylamide gel electrophoresis (PAGE). The gel incorporates a haloalkane which reacts with Trp and fluoresces under UV light. The haloalkane may be incorporated prior to polymerization of the gel or subsequently.
(FR)Selon la présente invention, la réaction induite par UV entre la fraction indole de tryptophane (Trp) et des haloalcanes permet la visualisation rapide de protéines séparées par électrophorèse en gel de polyacrylamide (PAGE). Ledit gel comprend un haloalcane qui réagit avec le Trp et fluoresce sous UV. Ledit haloalcane peut être incorporé préalablement à la polymérisation du gel ou ultérieurement.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PT, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)