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Machine translation
1. (WO2003054192) METHOD OF IDENTIFYING GIBBERELLIN RESPONSIVE GENE USING CULTURED VEGETABLE CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2003/054192    International Application No.:    PCT/JP2002/013376
Publication Date: 03.07.2003 International Filing Date: 20.12.2002
Chapter 2 Demand Filed:    18.06.2003    
IPC:
A01H 3/00 (2006.01), C07K 14/415 (2006.01), C12Q 1/68 (2006.01)
Applicants: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES [JP/JP]; 1-2, Kannondai 2-chome, Tsukuba-shi, Ibaraki 305 |8602 (JP) (For All Designated States Except US).
MINAMI, Eiichi [JP/JP]; (JP) (For US Only).
SHIBUYA, Naoto [JP/JP]; (JP) (For US Only).
DAY, Robert, B. [US/JP]; (JP) (For US Only)
Inventors: MINAMI, Eiichi; (JP).
SHIBUYA, Naoto; (JP).
DAY, Robert, B.; (JP)
Agent: SHIMIZU, Hatsushi; Kantetsu Tsukuba Bldg. 6F, 1-1-1, Oroshi-machi, Tsuchiura-shi, Ibaraki 300-0847 (JP)
Priority Data:
2001-388993 21.12.2001 JP
Title (EN) METHOD OF IDENTIFYING GIBBERELLIN RESPONSIVE GENE USING CULTURED VEGETABLE CELLS
(FR) PROCEDE D'IDENTIFICATION DE GENES SENSIBLES A LA GIBBERELLINE A L'AIDE DE CELLULES VEGETALES CULTIVEES
Abstract: front page image
(EN)It has never been considered in the art that cultured cells response to gibberellin. To comprehensively and conveniently identify and isolate gibberellin responsive genes, however, cultured cells which are uniform and easy to handle are appropriate. Considering these advantages of cultured cells, attempts are made to develop a method of identifying and isolating gibberellin responsive genes with the use of cultured cells. Cultured cells are pre-cultured in an auxin-free medium. Then, it is examined whether or not changes in gene expression are detected. As a result, it is surprisingly found out that some genes show changes in the expression after the gibberellin treatment.
(FR)Aucune étude antérieure n'a porté sur des cellules cultivées sensibles à la gibbérelline. Pour identifier et isoler de manière globale et pratique les gènes sensibles à la gibbérelline, il convient d'utiliser des cellules cultivées uniformes et facile à manipuler. Compte tenu des avantages des cellules cultivées, on a tenté de mettre sur pied un procédé d'identification et d'isolation des gènes sensibles à la gibbérelline qui fait intervenir des cellules cultivées. Les cellules cultivées ont d'abord été pré-cultivées dans un milieu exempt d'auxine. Que des changements de l'expression génique aient été détectés ou non, ces cellules ont toutes été examinées. Le résultat a montré, de façon surprenante, que l'expression de certains gènes subit un changement après le traitement à la gibbéreline.
Designated States: AU, CA, JP, US.
European Patent Office (DE, FR, GB, IT, NL, SI).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)